| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab191420-10μl |
10μl |
现货  |
| |
| Ab191420-50μl |
50μL |
现货 |
| |
| Ab191420-100μl |
100μL |
期货 |
| |
| Ab191420-1ml |
1ml |
期货 |
|
| 产品名称 | GBP6 Mouse mAb |
|---|---|
| 别名 | G蛋白结合蛋白6抗体 | GBP6抗体 | GBP6单克隆抗体 |
| 英文别名 | GBP-6 | GTP-binding protein 6 | Guanine nucleotide-binding protein 6 | guanylate binding protein family, member 6 | Guanylate-binding protein 6 |
| 规格或纯度 | ExactAb™, 无载体, 已验证, 1.0 mg/mL |
| 生化机理 | Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens, such as bacterial pathogens Listeria monocytogenes and Mycobacterium bovis BCG as well as the protozoan pathogen Toxoplasma gondii (By similarity). Confers protection to several pathogens, including the bacterial pathogens Listeria monocytogenes and Mycobacterium bovis BCG as well as the protozoan pathogen Toxoplasma gondii (By similarity). |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | GBP6 |
| 种属反应性 | 人(Human),小鼠(Mouse) |
| 免疫原 | Recombinant protein of human GBP6 (AA 291-570) |
| 阳性对照 | WB: Mouse liver, A431 lysates. IHC: Human esophagus tissue. |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 储存缓冲液 | 150mM PBS, 0.09% Sodium azide, pH 7.4 |
| 防腐剂 | 0.09% Sodium azide |
| 浓度 | 1.0 mg/mL |
| 储存温度 | -80℃储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| 分子类型 | 抗体 |
GBP6 Mouse mAb (Ab191420) - Western Blot
All lanes: GBP6 Mouse mAb (Ab191420) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 69 kDa
Exposure time: 70.5 s
GBP6 Mouse mAb (Ab191420) - Western Blot
All lanes: GBP6 Mouse mAb (Ab191420) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 69 kDa
Exposure time: 60.0 s
GBP6 Mouse mAb (Ab191420) - IHC
IHC analysis of esophagus tissue by GBP6 Mouse mAb (Ab191420).
IHC was performed using sections of the formalin-fixed paraffin-embedded esophagus tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with GBP6 Mouse mAb (Ab191420) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Squamous epithelial cells are positively stained at the cytoplasm.
| 应用名称 | 稀释比例 |
|---|---|
| WB |
1/1000
|
| IHC | 1/100 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F1214809 |
分析证书 | 24-12-04 | Ab191420 |
| ZJ24F1214808 |
分析证书 | 24-12-04 | Ab191420 |
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