产品名称	Vinculin Mouse mAb
别名	黏着斑蛋白抗体 \| 黏着斑蛋白 \| Anti-vinculin 抗体 \| 粘着斑蛋白小鼠mAb
英文别名	CMD1W antibody \| CMH15 antibody \| Epididymis luminal protein 114 antibody \| HEL114 antibody \| Metavinculin antibody \| MV antibody \| MVCL antibody \| OTTHUMP00000019861 antibody \| OTTHUMP00000019862 antibody \| VCL antibody \| VINC antibody \| VINC_HUMAN antib
规格或纯度	ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
宿主种属	小鼠(Mouse)
特异性	Vinculin
种属反应性	人(Human),小鼠(Mouse)
免疫原	Recombinant full length protein corresponding to Human Vinculin
阳性对照	WB: HeLa, HEK293, HepG2, A431 and NIH/3T3 whole cell lysates Flow: HeLa cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	AB04/5D4
Format	Whole IgG
亚型	IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, pH 7.2
防腐剂	No
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 +4°C 下短期储存（1-2 周）。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

图片

Western Blot: Vinculin Mouse mAb (Ab179928)

Vinculin Mouse mAb (Ab179928) - Western Blot
All lanes: Vinculin Antibody (Ab179928) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124.0 kDa
Observed band size: 129.7 kDa
Exposure time: 5.7 s

Flow Cytometry: Vinculin Mouse mAb (Ab179928)

Vinculin Mouse mAb (Ab179928) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab179928) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).