| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab177870-100μg |
100μg |
现货  |
| |
| Ab177870-1mg |
1mg |
现货 |
| |
| Ab177870-5mg |
5mg |
现货 |
| |
| Ab177870-10mg |
10mg |
期货 |
|
| 产品名称 | FR104 (anti-CD28) |
|---|---|
| 别名 | CD 28 antibody | CD28 antibody | CD28 antigen antibody | CD28 molecule antibody | CD28_HUMAN antibody | MGC138290 antibody | T cell antigen CD28 antibody | T cell specific surface glycoprotein antibody | T cell specific surface glycoprotein CD28 antibody |
| 英文别名 | TP44 |
| 规格或纯度 | 无载体, 重组, ExactAb™, 低内毒素, 无叠氮钠, 已验证, 无动物源, ≥95%(SDS-PAGE&SEC-HPLC), 见COA |
| 宿主种属 | 人(Human) |
| 特异性 | CD28 |
| 种属反应性 | 人(Human) |
| 偶联 | Unconjugated |
| 克隆类型 | 重组抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Human IgG2 |
| 轻链亚型 | kappa |
| SDS-PAGE | 26.1 kDa (Light Chain) & 51.1 kDa (Heavy Chain), under reducing conditions; 188.9 kDa, under non-reducing conditions. |
| 纯化方法 | Protein A purified |
| 纯度 | >95% |
| 物理外观 | Liquid |
| 储存缓冲液 | Supplied as a 0.22μm filtered solution in 100mM Pro-Ac, 20mM Arg, pH 5.0 |
| 防腐剂 | No |
| 浓度 | 见COA |
| 储存温度 | -80℃储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 -80℃ 下保存 24 个月。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
FR104 (anti-CD28) (Ab177870) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD28 with FR104 (anti-CD28) (Ab177870) conjugated with biotin (right panel) compared with Human IgG (Ab170213) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).
FR104 (anti-CD28) (Ab177870) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD28 (red) with FR104 (anti-CD28) (Ab177870) conjugated with biotin and detected with SA-PE (rp156150). Blue - Isotype control, human IgG (Ab170213) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
FR104 (anti-CD28) (Ab177870) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD28 (red) with FR104 (anti-CD28) (Ab177870). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
FR104 (anti-CD28) (Ab177870) - SEC
The purity of FR104 (anti-CD28) (Ab177870) is more than 95% verified by HPLC.
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F0404150 |
分析证书 | 24-04-03 | Ab177870 |
| ZJ24F0404149 |
分析证书 | 24-04-03 | Ab177870 |
| ZJ24F0404148 |
分析证书 | 24-04-03 | Ab177870 |
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| 1. Suchard SJ, Davis PM, Kansal S, Stetsko DK, Brosius R, Tamura J, Schneeweis L, Bryson J, Salcedo T, Wang H et al.. (2013) A monovalent anti-human CD28 domain antibody antagonist: preclinical efficacy and safety.. J Immunol, 191 (9): (4599-610). [PMID:24081989] |
| 2. Tomoko Hayashi, Ji-Hun Mo, Xing Gong, Cyprian Rossetto, Angela Jang, Lucinda Beck, Gregory I Elliott, Irina Kufareva, Ruben Abagyan, David H Broide, Jongdae Lee, Eyal Raz,. (2007-11-16) 3-Hydroxyanthranilic acid inhibits PDK1 activation and suppresses experimental asthma by inducing T cell apoptosis.. Proceedings of the National Academy of Sciences of the United States of America, 104 ((47)): ( 18619-18624 ). [PMID:18003900] |
| 3. Lee, K P KP and 5 more authors.. (1990) The genomic organization of the CD28 gene. Implications for the regulation of CD28 mRNA expression and heterogeneity.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2162892] |
| 4. Aruffo, A A and Seed, B B.. (1987) Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2825196] |
| 5. Blotta, M H MH, Marshall, J D JD, DeKruyff, R H RH and Umetsu, D T DT.. (1996) Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8617933] |
| 6. Ling, V V and 9 more authors.. (2001) Assembly and annotation of human chromosome 2q33 sequence containing the CD28, CTLA4, and ICOS gene cluster: analysis by computational, comparative, and microarray approaches.. Genomics, [PMID:11735222] |
| 7. Deshpande, Manisha M, Venuprasad, K K, Parab, Pradeep B PB, Saha, Bhaskar B and Mitra, Debashis D.. (2002) A novel CD28 mRNA variant and simultaneous presence of various CD28 mRNA isoforms in human T lymphocytes.. Human immunology, [PMID:11916166] |
| 8. Mikolajczak, Sebastian A SA and 5 more authors.. (2004) The modulation of CD40 ligand signaling by transmembrane CD28 splice variant in human T cells.. The Journal of experimental medicine, (5): [PMID:15067037] |
| 9. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186] |
| 10. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973] |
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