| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab135077-10μl |
10μl |
现货  |
| |
| Ab135077-50μl |
50μL |
现货 |
| |
| Ab135077-100μl |
100μL |
现货 |
| |
| Ab135077-1ml |
1ml |
期货 |
|
| 产品名称 | Recombinant YY1 Antibody |
|---|---|
| 别名 | 核转录调节因子YY1内参抗体 | 核转录调节因子抗体 | 核转录调节因子单克隆抗体 | 核转录调节因子重组单克隆抗体 | YY1抗体 | YY1单克隆抗体 | YY1重组单克隆抗体 |
| 英文别名 | Delta transcription factor | DELTA | INO80SYY-1 | NF-E1 | NF-E1INO80 complex subunit S | transcriptional repressor protein YY1 | UCRBP | Yin and Yang 1 protein | Yin and yang 1 | YIN-YANG-1 | YY1 transcription factor | YY1 | CF1 antibody | Delta antibody |
| 规格或纯度 | 重组, ExactAb™, 已验证, 见COA |
| 生化机理 | Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | YY1 |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 免疫原 | A synthetic peptide derived from human YY1 (AA 250-300). |
| 阳性对照 | WB: HEK293, A375, HeLa, K562, NIH/3T3 and C6 cell lysates. IHC: Human bladder tissue. IF/ICC: HeLa, MCF7 cells. |
| 偶联 | Unconjugated |
| 克隆类型 | 重组抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4 |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Recombinant YY1 Antibody (Ab135077) - Western Blot
All lanes: Recombinant YY1 Antibody (Ab135077) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 57 kDa
Exposure time: 45.6 s
Recombinant YY1 Antibody (Ab135077) - IHC
Immunohistochemical analysis of paraffin-embedded human bladder using
Recombinant YY1 Antibody (Ab135077).
Recombinant YY1 Antibody (Ab135077) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant YY1 Antibody (Ab135077) at 1/50 dilution.
Recombinant YY1 Antibody (Ab135077) - IF/ICC
Immunofluorescent analysis of MCF7 cells, using Recombinant YY1 Antibody (Ab135077) at 1/50 dilution.
Recombinant YY1 Antibody (Ab135077) - IF/ICC
Immunofluorescent analysis of MCF7 cells, using Recombinant YY1 Antibody (Ab135077) at 1/50 dilution.
Recombinant YY1 Antibody (Ab135077) - IF/ICC
Immunofluorescent analysis of MCF7 cells, using Recombinant YY1 Antibody (Ab135077) at 1/150 dilution.
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC |
1/50-1/200
|
| IHC | 1/100 - 1/200 |
| WB |
1/1000-1/2000
|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ25F0319350 |
分析证书 | 25-03-17 | Ab135077 |
| ZJ25F0319349 |
分析证书 | 25-03-17 | Ab135077 |
| ZJ25F0319348 |
分析证书 | 25-03-17 | Ab135077 |
¥139.90
| 1. Shi, Y Y, Seto, E E, Chang, L S LS and Shenk, T T.. (1991) Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein.. Cell, (18): [PMID:1655281] |
| 2. Park, K K and Atchison, M L ML.. (1991) Isolation of a candidate repressor/activator, NF-E1 (YY-1, delta), that binds to the immunoglobulin kappa 3' enhancer and the immunoglobulin heavy-chain mu E1 site.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1946405] |
| 3. Houbaviy, H B HB, Usheva, A A, Shenk, T T and Burley, S K SK.. (1996) Cocrystal structure of YY1 bound to the adeno-associated virus P5 initiator.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:8942976] |
| 4. Kalenik, J L JL, Chen, D D, Bradley, M E ME, Chen, S J SJ and Lee, T C TC.. (1997) Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1.. Nucleic acids research, (15): [PMID:9016636] |
| 5. McNeil, S S and 9 more authors.. (1998) Targeting of the YY1 transcription factor to the nucleolus and the nuclear matrix in situ: the C-terminus is a principal determinant for nuclear trafficking.. Journal of cellular biochemistry, (15): [PMID:9493912] |
| 6. Viles, J H JH and 9 more authors.. (1998) Design, synthesis and structure of a zinc finger with an artificial beta-turn.. Journal of molecular biology, (19): [PMID:9642075] |
| 7. Satijn, D P DP, Hamer, K M KM, den Blaauwen , J J and Otte, A P AP.. (2001) The polycomb group protein EED interacts with YY1, and both proteins induce neural tissue in Xenopus embryos.. Molecular and cellular biology, [PMID:11158321] |
| 8. Lee, Kyu-Ho KH, Evans, Samuel S, Ruan, Todd Y TY and Lassar, Andrew B AB.. (2004) SMAD-mediated modulation of YY1 activity regulates the BMP response and cardiac-specific expression of a GATA4/5/6-dependent chick Nkx2.5 enhancer.. Development (Cambridge, England), [PMID:15329343] |
| 9. Gordon, S S, Akopyan, G G, Garban, H H and Bonavida, B B.. (2006) Transcription factor YY1: structure, function, and therapeutic implications in cancer biology.. Oncogene, (23): [PMID:16314846] |
| 10. Cai, Yong Y and 10 more authors.. (2007) YY1 functions with INO80 to activate transcription.. Nature structural & molecular biology, [PMID:17721549] |
| 11. Wu, Su S and 11 more authors.. (2007) A YY1-INO80 complex regulates genomic stability through homologous recombination-based repair.. Nature structural & molecular biology, [PMID:18026119] |
| 12. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 13. Yao, Tingting T and 10 more authors.. (2008) Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex.. Molecular cell, (26): [PMID:18922472] |
| 14. Kim, Jeong do Jd and Kim, Joomyeong J.. (2009) YY1's longer DNA-binding motifs.. Genomics, [PMID:18950698] |
| 15. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 16. Del Rincón, Sonia V and 5 more authors.. (2010) Development and validation of a method for profiling post-translational modification activities using protein microarrays.. PloS one, (28): [PMID:20596523] |
| 17. Vissers, Lisenka E L M LE and 14 more authors.. (2010) A de novo paradigm for mental retardation.. Nature genetics, [PMID:21076407] |
| 18. Chen, Lu L and 7 more authors.. (2011) Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling.. The Journal of biological chemistry, (1): [PMID:21303910] |
| 19. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 20. Riman, Sarah S and 5 more authors.. (2012) Phosphorylation of the transcription factor YY1 by CK2α prevents cleavage by caspase 7 during apoptosis.. Molecular and cellular biology, [PMID:22184066] |
| 21. Lee, Kwanghyun K, Na, Wonho W, Maeng, Je-Heon JH, Wu, Hongjin H and Ju, Bong-Gun BG.. (2013) Regulation of DU145 prostate cancer cell growth by Scm-like with four mbt domains 2.. Journal of biosciences, [PMID:23385818] |
| 22. Cao, Yanan Y and 19 more authors.. (2013) Whole exome sequencing of insulinoma reveals recurrent T372R mutations in YY1.. Nature communications, [PMID:24326773] |
| 23. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364] |
| 24. Cromer, M Kyle MK and 16 more authors.. (2015) Neomorphic effects of recurrent somatic mutations in Yin Yang 1 in insulin-producing adenomas.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:25787250] |
| 25. Gabriele, Michele M and 50 more authors.. (2017) YY1 Haploinsufficiency Causes an Intellectual Disability Syndrome Featuring Transcriptional and Chromatin Dysfunction.. American journal of human genetics, (1): [PMID:28575647] |
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