| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab124351-10μl |
10μl |
现货  |
| |
| Ab124351-50μl |
50μL |
现货 |
| |
| Ab124351-100μl |
100μL |
现货 |
| |
| Ab124351-1ml |
1ml |
期货 |
|
| 产品名称 | Recombinant Rad21 Antibody |
|---|---|
| 别名 | Anti-Rad21抗体 | 核基质蛋白21抗体 | 重组Rad21抗体 |
| 英文别名 | CDLS4 antibody | Double-strand-break repair protein rad21 homolog antibody | hHR21 antibody | HR21 antibody | HRAD21 antibody | KIAA0078 antibody | MCD1 antibody | Nuclear matrix protein 1 antibody | NXP-1 antibody | NXP1 antibody | Protein involved in DN |
| 规格或纯度 | ExactAb™, Validated, 重组, 0.6 mg/mL |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | RAD21 |
| 种属反应性 | 人(Human),仓鼠(Hamster) |
| 免疫原 | A synthetic peptide derived from human Rad21 (AA 600-631). |
| 阳性对照 | WB: Hela, A549, K562 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cell. |
| 偶联 | Unconjugated |
| 克隆类型 | 重组抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kD |
| 纯化方法 | Antigen affinity purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4 |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.6 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Recombinant Rad21 Antibody (Ab124351) - Western Blot
All lanes: Recombinant Rad21 Antibody (Ab124351) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 130 kDa
Observed band size: 117 kDa
Exposure time: 5.8 s
Recombinant Rad21 Antibody (Ab124351) - ICC/IF
Immunocytochemistry analysis of Rad21 (green) in HeLa cells using Recombinant Rad21 Antibody (Ab124351) and cells were counterstained with DAPI (blue).
Recombinant Rad21 Antibody (Ab124351) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant Rad21 Antibody (Ab124351). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| 应用名称 | 稀释比例 |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
| IF/ICC |
1/50-1/200
|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F0911283 |
分析证书 | 24-09-27 | Ab124351 |
| ZJ23F0800849 |
分析证书 | 23-08-17 | Ab124351 |
| ZJ23F0800850 |
分析证书 | 23-08-17 | Ab124351 |
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| 2. McKay, M J MJ and 7 more authors.. (1996) Sequence conservation of the rad21 Schizosaccharomyces pombe DNA double-strand break repair gene in human and mouse.. Genomics, (1): [PMID:8812457] |
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| 8. Gregson, H C HC and 5 more authors.. (2001) A potential role for human cohesin in mitotic spindle aster assembly.. The Journal of biological chemistry, (14): [PMID:11590136] |
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| 13. Rankin, Susannah S, Ayad, Nagi G NG and Kirschner, Marc W MW.. (2005) Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates.. Molecular cell, (15): [PMID:15837422] |
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| 17. Bermudez, Vladimir P VP and 6 more authors.. (2012) In vitro loading of human cohesin on DNA by the human Scc2-Scc4 loader complex.. Proceedings of the National Academy of Sciences of the United States of America, (12): [PMID:22628566] |
| 18. Deardorff, Matthew A MA and 26 more authors.. (2012) RAD21 mutations cause a human cohesinopathy.. American journal of human genetics, (8): [PMID:22633399] |
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| 20. Kruszka, Paul P and 34 more authors.. (2019) Cohesin complex-associated holoprosencephaly.. Brain : a journal of neurology, (23): [PMID:31334757] |
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