| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab121687-10μl |
10μl |
现货  |
| |
| Ab121687-50μl |
50μL |
现货 |
| |
| Ab121687-100μl |
100μL |
期货 |
| |
| Ab121687-1ml |
1ml |
期货 |
|
| 产品名称 | Recombinant Phospholipase C gamma 1/PLC-gamma-1 Antibody |
|---|---|
| 别名 | 磷酯酶Cγ1抗体 | 磷酯酶Cγ1抗体 | PLCG1抗体 | PLCG1单抗 | PLCG1兔单抗 | PLCG1重组兔单抗 |
| 英文别名 | EC 3.1.4.11 | gamma 1 (formerly subtype 148) | monophosphatidylinositol phosphodiesterase | NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 | phosphatidylinositol phospholipase C | phosphoinositidase C | phosphoinositide phospholi |
| 规格或纯度 | 重组, ExactAb™, 已验证, 见COA |
| 生化机理 | Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | PLCG1 |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 预测反应种属 | 牛(Cow) |
| 免疫原 | A synthetic peptide derived from human PLCG1 (AA 750-810). |
| 阳性对照 | WB: Rat brain, HeLa, SH-SY5Y, Hep G2 and Jurkat lysates. IF/ICC: HeLa cells. |
| 偶联 | Unconjugated |
| 克隆类型 | 重组抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4 |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Recombinant Phospholipase C gamma 1/PLC-gamma-1 Antibody (Ab121687) - Western Blot
All lanes: Recombinant Phospholipase C gamma 1/PLC-gamma-1 Antibody (Ab121687) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 149 kDa
Observed band size: 150 kDa
Exposure time: 114.2 s
Recombinant Phospholipase C gamma 1/PLC-gamma-1 Antibody (Ab121687) - Western Blot
All lanes: Recombinant Phospholipase C gamma 1/PLC-gamma-1 Antibody (Ab121687) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 150 kDa
Exposure time: 100.0 s
Recombinant Phospholipase C gamma 1/PLC-gamma-1 Antibody (Ab121687) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Phospholipase C gamma 1/PLC-gamma-1 Antibody (Ab121687) at 1/50 dilution.
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC | 1/50 - 1/200 |
| WB | 1/1000 - 1/2000 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F1215947 |
分析证书 | 24-12-24 | Ab121687 |
| ZJ24F1215946 |
分析证书 | 24-12-24 | Ab121687 |
| 1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Huang MR, Hsu YL, Lin TC, Cheng TJ, Li LW, Tseng YW, Chou YS, Liu JH, Pan SH, Fang JM, Wong CH.. (2019) Structure-guided development of purine amide, hydroxamate, and amidoxime for the inhibition of non-small cell lung cancer.. Eur J Med Chem, 181 (111551-111551). [PMID:31376567] [10.1016/j.ejmech.2019.07.054] |
| 4. Nguyen HT, Guégan JP, Poissonnier A, Jouan F, Best D, van de Weghe P, Vacher P, Levoin N, Legembre P, Jean M.. (2019) Synthesis of peptidomimetics and chemo-biological tools for CD95/PLCγ1 interaction analysis.. Bioorg Med Chem Lett, 29 (16): (2094-2099). [PMID:31301931] [10.1016/j.bmcl.2019.07.006] |
| 5. Mohammadi, M M and 9 more authors.. (1991) A tyrosine-phosphorylated carboxy-terminal peptide of the fibroblast growth factor receptor (Flg) is a binding site for the SH2 domain of phospholipase C-gamma 1.. Molecular and cellular biology, [PMID:1656221] |
| 6. Burgess, W H WH and 7 more authors.. (1990) Characterization and cDNA cloning of phospholipase C-gamma, a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.. Molecular and cellular biology, [PMID:2167438] |
| 7. Vainikka, S S and 5 more authors.. (1994) Signal transduction by fibroblast growth factor receptor-4 (FGFR-4). Comparison with FGFR-1.. The Journal of biological chemistry, (15): [PMID:7518429] |
| 8. Kohda, D D and 6 more authors.. (1993) Solution structure of the SH3 domain of phospholipase C-gamma.. Cell, (26): [PMID:7681365] |
| 9. Law, C L CL, Chandran, K A KA, Sidorenko, S P SP and Clark, E A EA.. (1996) Phospholipase C-gamma1 interacts with conserved phosphotyrosyl residues in the linker region of Syk and is a substrate for Syk.. Molecular and cellular biology, [PMID:8657103] |
| 10. Price, D J DJ and 5 more authors.. (1997) Direct association of Csk homologous kinase (CHK) with the diphosphorylated site Tyr568/570 of the activated c-KIT in megakaryocytes.. The Journal of biological chemistry, (28): [PMID:9038210] |
| 11. Braunger, J J and 7 more authors.. (1997) Intracellular signaling of the Ufo/Axl receptor tyrosine kinase is mediated mainly by a multi-substrate docking-site.. Oncogene, (5): [PMID:9178760] |
| 12. Zhang, W W, Sloan-Lancaster, J J, Kitchen, J J, Trible, R P RP and Samelson, L E LE.. (1998) LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.. Cell, (9): [PMID:9489702] |
| 13. Dougher, M M and Terman, B I BI.. (1999) Autophosphorylation of KDR in the kinase domain is required for maximal VEGF-stimulated kinase activity and receptor internalization.. Oncogene, (25): [PMID:10102632] |
| 14. Lindholm, C K CK, Gylfe, E E, Zhang, W W, Samelson, L E LE and Welsh, M M.. (1999) Requirement of the Src homology 2 domain protein Shb for T cell receptor-dependent activation of the interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells.. The Journal of biological chemistry, (24): [PMID:10488157] |
| 15. Rebhun, J F JF, Chen, H H and Quilliam, L A LA.. (2000) Identification and characterization of a new family of guanine nucleotide exchange factors for the ras-related GTPase Ral.. The Journal of biological chemistry, (5): [PMID:10747847] |
| 16. Felschow, D M DM, Civin, C I CI and Hoehn, G T GT.. (2000) Characterization of the tyrosine kinase Tnk1 and its binding with phospholipase C-gamma1.. Biochemical and biophysical research communications, (24): [PMID:10873601] |
| 17. Baker, J E JE, Majeti, R R, Tangye, S G SG and Weiss, A A.. (2001) Protein tyrosine phosphatase CD148-mediated inhibition of T-cell receptor signal transduction is associated with reduced LAT and phospholipase Cgamma1 phosphorylation.. Molecular and cellular biology, [PMID:11259588] |
| 18. Korkaya, H H and 11 more authors.. (2001) The ORF3 protein of hepatitis E virus binds to Src homology 3 domains and activates MAPK.. The Journal of biological chemistry, (9): [PMID:11518702] |
| 19. Lin, Joseph J and Weiss, Arthur A.. (2003) The tyrosine phosphatase CD148 is excluded from the immunologic synapse and down-regulates prolonged T cell signaling.. The Journal of cell biology, (18): [PMID:12913111] |
| 20. Wang, Jian Feng JF, Zhang, Xuefeng X and Groopman, Jerome E JE.. (2004) Activation of vascular endothelial growth factor receptor-3 and its downstream signaling promote cell survival under oxidative stress.. The Journal of biological chemistry, (25): [PMID:15102829] |
| 21. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186] |
| 22. Jia, Haiyan H and 5 more authors.. (2004) Vascular endothelial growth factor (VEGF)-D and VEGF-A differentially regulate KDR-mediated signaling and biological function in vascular endothelial cells.. The Journal of biological chemistry, (20): [PMID:15215251] |
| 23. Taguchi, Tomoko T and 14 more authors.. (2004) Deficiency of BLNK hampers PLC-gamma2 phosphorylation and Ca2+ influx induced by the pre-B-cell receptor in human pre-B cells.. Immunology, [PMID:15270728] |
| 24. Tacconelli, Antonella A and 11 more authors.. (2004) TrkA alternative splicing: a regulated tumor-promoting switch in human neuroblastoma.. Cancer cell, [PMID:15488758] |
| 25. Rönnstrand, L L.. (2004) Signal transduction via the stem cell factor receptor/c-Kit.. Cellular and molecular life sciences : CMLS, [PMID:15526160] |
| 26. Eswarakumar, V P VP, Lax, I I and Schlessinger, J J.. (2005) Cellular signaling by fibroblast growth factor receptors.. Cytokine & growth factor reviews, [PMID:15863030] |
| 27. Hatch, Nan E NE, Hudson, Mark M, Seto, Marianne L ML, Cunningham, Michael L ML and Bothwell, Mark M.. (2006) Intracellular retention, degradation, and signaling of glycosylation-deficient FGFR2 and craniosynostosis syndrome-associated FGFR2C278F.. The Journal of biological chemistry, (15): [PMID:16844695] |
| 28. Wang, Yaohong Y, Tomar, Alok A, George, Sudeep P SP and Khurana, Seema S.. (2007) Obligatory role for phospholipase C-gamma(1) in villin-induced epithelial cell migration.. American journal of physiology. Cell physiology, [PMID:17229814] |
| 29. Harada, Daisuke D and 6 more authors.. (2007) Sustained phosphorylation of mutated FGFR3 is a crucial feature of genetic dwarfism and induces apoptosis in the ATDC5 chondrogenic cell line via PLCgamma-activated STAT1.. Bone, [PMID:17561467] |
| 30. Reddi, Alagarsamy Lakku AL and 9 more authors.. (2007) Binding of Cbl to a phospholipase Cgamma1-docking site on platelet-derived growth factor receptor beta provides a dual mechanism of negative regulation.. The Journal of biological chemistry, (5): [PMID:17620338] |
| 31. Salazar, Lisa L and 6 more authors.. (2009) A novel interaction between fibroblast growth factor receptor 3 and the p85 subunit of phosphoinositide 3-kinase: activation-dependent regulation of ERK by p85 in multiple myeloma cells.. Human molecular genetics, (1): [PMID:19286672] |
| 32. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 33. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 34. Persaud, Avinash A and 8 more authors.. (2011) Nedd4-1 binds and ubiquitylates activated FGFR1 to control its endocytosis and function.. The EMBO journal, (15): [PMID:21765395] |
| 35. Wang, Di D and 16 more authors.. (2012) Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling.. Nature immunology, (6): [PMID:22561606] |
| 36. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
首页
400-620-6333
