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Recombinant PHD2/prolyl hydroxylase Antibody

Recombinant PHD2/prolyl hydroxylase Antibody

级别和纯度:

0.6 mg/mL

应用:

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab121566-10μl	10μl	现货
Ab121566-50μl	50μL	现货
Ab121566-100μl	100μL	期货
Ab121566-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: EGLN1

Accession#:Q9GZT9 (1) EGLN1 (1) Gene ID:54583 (1) PHD2 (1) prolyl hydroxylase (2)

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基本信息

产品名称	Recombinant PHD2/prolyl hydroxylase Antibody
别名	Egl9同源物1(EGLN1)抗体 \| PHD2/prolyl hydroxylase抗体 \| 重组PHD2/脯氨酰羟化酶抗体
英文别名	C1orf12 \| EC 1.14.11.29 \| EC:1.14.11.291 \| ECYT3 \| egl nine homolog 1 \| egl nine-like protein 1 \| EGLN1 \| HALAH \| HIF prolyl hydroxylase 2 \| HIFPH2 \| HIF-PH2 \| HIF-prolyl hydroxylase 2 \| HPH2 \| HPH-2 \| Hypoxia-inducible factor prolyl hydroxylase 2 \| PHD2
规格或纯度	ExactAb™, Validated, 重组, 0.6 mg/mL
生化机理	Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
宿主种属	兔(Rabbit)
特异性	PHD2/prolyl hydroxylase
种属反应性	人(Human),小鼠(Mouse)
免疫原	A synthetic peptide derived from human PHD2/prolyl hydroxylase (AA 1-51)
阳性对照	WB: Mouse kidney lysates.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Rabbit IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂	0.02% Sodium azide
浓度	0.6 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

EGLN1 Tclin Egl nine 同源物 1(Egl nine homolog 1) (0 活性数据)

点击查看靶蛋白信息： EGLN1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Recombinant PHD2/prolyl hydroxylase Antibody (Ab121566)

Recombinant PHD2/prolyl hydroxylase Antibody (Ab121566) - Western Blot
All lanes: Recombinant PHD2/prolyl hydroxylase Antibody (Ab121566) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 47 kDa
Exposure time: 256.6 s

应用

应用名称	稀释比例
WB	1/500-1/2000

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找到2个结果

批号(Lot Number)	证书类型	日期	货号
ZJ24F0505721	分析证书	24-05-16	Ab121566
ZJ24F0505720	分析证书	24-05-16	Ab121566

技术文档和文章

高分子量蛋白质印迹法

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免疫印迹生物缓冲液蛋白质印迹高分子量蛋白大分子蛋白蛋白质电泳 WB抗体

Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

Tags:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

Tags:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

Tags:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

此产品的引用文献

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引用文献

1. Rose NR, Woon EC, Tumber A, Walport LJ, Chowdhury R, Li XS, King ON, Lejeune C, Ng SS, Krojer T et al.. (2012) Plant growth regulator daminozide is a selective inhibitor of human KDM2/7 histone demethylases.. J Med Chem, 55 (14): (6639-43). [PMID:22724510]
2. Semenza GL. (2001) HIF-1, O(2), and the 3 PHDs: how animal cells signal hypoxia to the nucleus.. Cell, 107 (1): (1-3). [PMID:11595178]
3. Schiller R, Scozzafava G, Tumber A, Wickens JR, Bush JT, Rai G, Lejeune C, Choi H, Yeh TL, Chan MC et al.. (2014) A cell-permeable ester derivative of the JmjC histone demethylase inhibitor IOX1.. ChemMedChem, 9 (3): (566-71). [PMID:24504543]
4. Vachal P, Miao S, Pierce JM, Guiadeen D, Colandrea VJ, Wyvratt MJ, Salowe SP, Sonatore LM, Milligan JA, Hajdu R et al.. (2012) 1,3,8-Triazaspiro[4.5]decane-2,4-diones as efficacious pan-inhibitors of hypoxia-inducible factor prolyl hydroxylase 1-3 (HIF PHD1-3) for the treatment of anemia.. J Med Chem, 55 (7): (2945-59). [PMID:22364528]
5. Ogoshi Y, Matsui T, Mitani I, Yokota M, Terashita M, Motoda D, Ueyama K, Hotta T, Ito T, Hase Y et al.. (2017) Discovery of JTZ-951: A HIF Prolyl Hydroxylase Inhibitor for the Treatment of Renal Anemia.. ACS Med Chem Lett, 8 (12): (1320-1325). [PMID:29259755]
6. Yeh TL, Leissing TM, Abboud MI, Thinnes CC, Atasoylu O, Holt-Martyn JP, Zhang D, Tumber A, Lippl K, Lohans CT et al.. (2017) Molecular and cellular mechanisms of HIF prolyl hydroxylase inhibitors in clinical trials.. Chem Sci, 8 (11): (7651-7668). [PMID:29435217]
7. Joharapurkar AA, Pandya VB, Patel VJ, Desai RC, Jain MR. (2018) Prolyl Hydroxylase Inhibitors: A Breakthrough in the Therapy of Anemia Associated with Chronic Diseases.. J Med Chem, 61 (16): (6964-6982). [PMID:29712435]
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9. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
10. Rotili D, Tomassi S, Conte M, Benedetti R, Tortorici M, Ciossani G, Valente S, Marrocco B, Labella D, Novellino E, Mattevi A, Altucci L, Tumber A, Yapp C, King ON, Hopkinson RJ, Kawamura A, Schofield CJ, Mai A.. (2014) Pan-histone demethylase inhibitors simultaneously targeting Jumonji C and lysine-specific demethylases display high anticancer activities.. J Med Chem, 57 (1): (42-55). [PMID:24325601] [10.1021/jm4012802]
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12. Lei Y, Hu T, Wu X, Wu Y, Bao Q, Zhang L, Xia H, Sun H, You Q, Zhang X.. (2015) Affinity-Based Fluorescence Polarization Assay for High-Throughput Screening of Prolyl Hydroxylase 2 Inhibitors.. ACS Med Chem Lett, 6 (12): (1236-1240). [PMID:26713111] [10.1021/acsmedchemlett.5b00394]
13. Ahmed S, Ayscough A, Barker GR, Canning HE, Davenport R, Downham R, Harrison D, Jenkins K, Kinsella N, Livermore DG, Wright S, Ivetac AD, Skene R, Wilkens SJ, Webster NA, Hendrick AG.. (2017) 1,2,4-Triazolo-[1,5-a]pyridine HIF Prolylhydroxylase Domain-1 (PHD-1) Inhibitors With a Novel Monodentate Binding Interaction.. J Med Chem, 60 (13): (5663-5672). [PMID:28594552] [10.1021/acs.jmedchem.7b00352]
14. Hamada M, Takayama T, Shibata T, Hiratate A, Takahashi M, Yashiro M, Takayama N, Okumura-Kitajima L, Koretsune H, Kajiyama H, Naruse T, Kato S, Takano H, Kakinuma H.. (2018) Discovery of novel 2-[(4-hydroxy-6-oxo-2,3-dihydro-1H-pyridine-5-carbonyl)amino]acetic acid derivatives as HIF prolyl hydroxylase inhibitors for treatment of renal anemia.. Bioorg Med Chem Lett, 28 (10): (1725-1730). [PMID:29681433] [10.1016/j.bmcl.2018.04.039]
15. Wu Y, Jiang Z, Li Z, Gu J, You Q, Zhang X.. (2018) Click Chemistry-Based Discovery of [3-Hydroxy-5-(1 H-1,2,3-triazol-4-yl)picolinoyl]glycines as Orally Active Hypoxia-Inducing Factor Prolyl Hydroxylase Inhibitors with Favorable Safety Profiles for the Treatment of Anemia.. J Med Chem, 61 (12): (5332-5349). [PMID:29856623] [10.1021/acs.jmedchem.8b00549]
16. Li Z, Su K, Jiang Z, Yu Y, You Q, Zhang X.. (2019) Photoactivatable Prolyl Hydroxylase 2 Inhibitors for Stabilizing the Hypoxia-Inducible Factor with Light.. J Med Chem, 62 (16): (7583-7588). [PMID:31244107] [10.1021/acs.jmedchem.9b00688]
17. Holt-Martyn JP, Tumber A, Rahman MZ, Lippl K, Figg W, Figg W, McDonough MA, Chowdhury R, Schofield CJ.. (2019) Studies on spiro[4.5]decanone prolyl hydroxylase domain inhibitors.. Medchemcomm, 10 (4): (500-504). [PMID:31057728] [10.1039/C8MD00548F]
18. Zhang X,Lei Y,Hu T,Wu Y,Li Z,Jiang Z,Yang C,Zhang L,You Q. (2020) Discovery of Clinical Candidate (5-(3-(4-Chlorophenoxy)prop-1-yn-1-yl)-3-hydroxypicolinoyl)glycine, an Orally Bioavailable Prolyl Hydroxylase Inhibitor for the Treatment of Anemia.. J Med Chem, 63 (17.0): (10045-10060). [PMID:32787144] [10.1021/acs.jmedchem.0c01161]
19. Debenham JS,Madsen-Duggan C,Clements MJ,Walsh TF,Kuethe JT,Reibarkh M,Salowe SP,Sonatore LM,Hajdu R,Milligan JA,Visco DM,Zhou D,Lingham RB,Stickens D,DeMartino JA,Tong X,Wolff M,Pang J,Miller RR,Sherer EC,Hale JJ. (2016) Discovery of N-[Bis(4-methoxyphenyl)methyl]-4-hydroxy-2-(pyridazin-3-yl)pyrimidine-5-carboxamide (MK-8617), an Orally Active Pan-Inhibitor of Hypoxia-Inducible Factor Prolyl Hydroxylase 1-3 (HIF PHD1-3) for the Treatment of Anemia.. J Med Chem, 59 (24.0): (11039-11049). [PMID:28002958] [10.1021/acs.jmedchem.6b01242]
20. Dupuy, D D and 7 more authors.. (2000) Mapping, characterization, and expression analysis of the SM-20 human homologue, c1orf12, and identification of a novel related gene, SCAND2.. Genomics, (1): [PMID:11056053]
21. and Taylor, M S MS.. (2001) Characterization and comparative analysis of the EGLN gene family.. Gene, (5): [PMID:11574160]
22. Epstein, A C AC and 19 more authors.. (2001) C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation.. Cell, (5): [PMID:11595184]
23. Oehme, Felix F and 7 more authors.. (2002) Overexpression of PH-4, a novel putative proline 4-hydroxylase, modulates activity of hypoxia-inducible transcription factors.. Biochemical and biophysical research communications, (16): [PMID:12163023]
24. Ivan, Mircea M and 10 more authors.. (2002) Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:12351678]
25. Metzen, Eric E and 11 more authors.. (2003) Intracellular localisation of human HIF-1 alpha hydroxylases: implications for oxygen sensing.. Journal of cell science, (1): [PMID:12615973]
26. Cioffi, Catherine L CL, Liu, Xiao Qin XQ, Kosinski, Penelope A PA, Garay, Michelle M and Bowen, Benjamin R BR.. (2003) Differential regulation of HIF-1 alpha prolyl-4-hydroxylase genes by hypoxia in human cardiovascular cells.. Biochemical and biophysical research communications, (11): [PMID:12670503]
27. Hirsilä, Maija M, Koivunen, Peppi P, Günzler, Volkmar V, Kivirikko, Kari I KI and Myllyharju, Johanna J.. (2003) Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor.. The Journal of biological chemistry, (15): [PMID:12788921]
28. Appelhoff, Rebecca J RJ and 7 more authors.. (2004) Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.. The Journal of biological chemistry, (10): [PMID:15247232]
29. Ozer, Abdullah A, Wu, Leeju C LC and Bruick, Richard K RK.. (2005) The candidate tumor suppressor ING4 represses activation of the hypoxia inducible factor (HIF).. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:15897452]
30. Percy, Melanie J MJ and 7 more authors.. (2006) A family with erythrocytosis establishes a role for prolyl hydroxylase domain protein 2 in oxygen homeostasis.. Proceedings of the National Academy of Sciences of the United States of America, (17): [PMID:16407130]
31. Davidson, Todd L TL, Chen, Haobin H, Di Toro, Dominic M DM, D'Angelo, Gisela G and Costa, Max M.. (2006) Soluble nickel inhibits HIF-prolyl-hydroxylases creating persistent hypoxic signaling in A549 cells.. Molecular carcinogenesis, [PMID:16649251]
32. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
33. McDonough, Michael A MA and 16 more authors.. (2006) Cellular oxygen sensing: Crystal structure of hypoxia-inducible factor prolyl hydroxylase (PHD2).. Proceedings of the National Academy of Sciences of the United States of America, (27): [PMID:16782814]
34. Percy, Melanie J MJ and 5 more authors.. (2007) A novel erythrocytosis-associated PHD2 mutation suggests the location of a HIF binding groove.. Blood, (15): [PMID:17579185]
35. Furlow, Paul W PW and 7 more authors.. (2009) Erythrocytosis-associated HIF-2alpha mutations demonstrate a critical role for residues C-terminal to the hydroxylacceptor proline.. The Journal of biological chemistry, (3): [PMID:19208626]
36. Yasumoto, Ken-ichi K, Kowata, Yusuke Y, Yoshida, Atsushi A, Torii, Satoru S and Sogawa, Kazuhiro K.. (2009) Role of the intracellular localization of HIF-prolyl hydroxylases.. Biochimica et biophysica acta, [PMID:19339211]
37. Chowdhury, Rasheduzzaman R and 7 more authors.. (2009) Structural basis for binding of hypoxia-inducible factor to the oxygen-sensing prolyl hydroxylases.. Structure (London, England : 1993), (15): [PMID:19604478]
38. Steinhoff, Amrei A and 6 more authors.. (2009) Cellular oxygen sensing: Importins and exportins are mediators of intracellular localisation of prolyl-4-hydroxylases PHD1 and PHD2.. Biochemical and biophysical research communications, (2): [PMID:19631610]
39. Simonson, Tatum S TS and 11 more authors.. (2010) Genetic evidence for high-altitude adaptation in Tibet.. Science (New York, N.Y.), (2): [PMID:20466884]
40. Bigham, Abigail and 13 more authors.. (2010) Identifying signatures of natural selection in Tibetan and Andean populations using dense genome scan data.. PLoS genetics, (9): [PMID:20838600]
41. Flashman, Emily E and 5 more authors.. (2010) Evidence for the slow reaction of hypoxia-inducible factor prolyl hydroxylase 2 with oxygen.. The FEBS journal, [PMID:20840591]
42. Chowdhury, Rasheduzzaman R and 9 more authors.. (2011) Studies on the reaction of nitric oxide with the hypoxia-inducible factor prolyl hydroxylase domain 2 (EGLN1).. Journal of molecular biology, (8): [PMID:21601578]
43. Su, Yun Y and 7 more authors.. (2012) Prolyl hydroxylase-2 (PHD2) exerts tumor-suppressive activity in pancreatic cancer.. Cancer, (15): [PMID:21792862]
44. Foxler, Daniel E DE and 13 more authors.. (2012) The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity.. Nature cell biology, (29): [PMID:22286099]
45. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
46. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
47. Seo, K-S KS and 14 more authors.. (2015) SIRT2 regulates tumour hypoxia response by promoting HIF-1α hydroxylation.. Oncogene, (12): [PMID:24681946]
48. Song, Daisheng D and 7 more authors.. (2014) Defective Tibetan PHD2 binding to p23 links high altitude adaption to altered oxygen sensing.. The Journal of biological chemistry, (23): [PMID:24711448]
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51. Tumber, Anthony A and 27 more authors.. (2017) Potent and Selective KDM5 Inhibitor Stops Cellular Demethylation of H3K4me3 at Transcription Start Sites and Proliferation of MM1S Myeloma Cells.. Cell chemical biology, (16): [PMID:28262558]
52. and Smirnoff, Nicholas.. (2018) Ascorbic acid metabolism and functions: A comparison of plants and mammals.. Free radical biology & medicine, [PMID:29567393]

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Recombinant PHD2/prolyl hydroxylase Antibody

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基本信息

产品属性

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