PGM1 Antibody

    应用:
  • IHC
  • WB
功能和特点
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
有货

库存信息

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库存信息

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库存信息

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库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Ab121491-10μl
10μl 现货 Stock Image
Ab121491-50μl
50μL 现货 Stock Image
Ab121491-100μl
100μL 现货 Stock Image
Ab121491-1ml
1ml 期货 Stock Image

基本信息

产品名称 PGM1 Antibody
别名 葡萄糖磷酸变位酶1多克隆抗体 | 葡萄糖磷酸变位酶1抗体 | PGM1抗体
英文别名 EC 5.4.2.2 | Glucose phosphomutase 1 | PGM 1 | CDG1T antibody | Glucose phosphomutase 1 antibody | GSD14 antibody | OTTHUMP00000010519 antibody | OTTHUMP00000010520 antibody | PGM 1 antibody | PGM1 antibody | PGM1_HUMAN antibody | Phosphoglucomutase 1 ant
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属 兔(Rabbit)
特异性 PGM1
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 Recombinant PGM1 expressed in E.coli (AA 1-235).
阳性对照 WB: HeLa, HEK293, A431, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human cerebrum, liver, kidney, stomach, breast cancer, prostate tissues.
偶联 Unconjugated

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Antigen affinity purified + Protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

PGM1 Antibody (Ab121491) - Western Blot
All lanes: PGM1 Antibody (Ab121491) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 65.9 s

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Sample: Human Liver Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Sample: Human Prostate Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

关联靶点(人)

PGM1 Tbio 磷酸葡糖变位酶-1(Phosphoglucomutase-1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

安全和危险性(GHS)

质量标准

应用

应用名称 稀释比例
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到3个结果

批号(Lot Number) 证书类型 日期 货号
ZJ24F0404498 分析证书 24-04-15 Ab121491
ZJ24F0404497 分析证书 24-04-15 Ab121491
ZJ24F0404496 分析证书 24-04-15 Ab121491

参考文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Whitehouse, D B DB and 7 more authors..  (1992)  Phosphoglucomutase 1: complete human and rabbit mRNA sequences and direct mapping of this highly polymorphic marker on human chromosome 1..  Proceedings of the National Academy of Sciences of the United States of America,  (1): [PMID:1530890]
3. Takahashi, N N and Neel, J V JV..  (1993)  Intragenic recombination at the human phosphoglucomutase 1 locus: predictions fulfilled..  Proceedings of the National Academy of Sciences of the United States of America,  (15): [PMID:7902567]
4. March, R E RE and 7 more authors..  (1993)  The classical human phosphoglucomutase (PGM1) isozyme polymorphism is generated by intragenic recombination..  Proceedings of the National Academy of Sciences of the United States of America,  (15): [PMID:7902568]
5. Putt, W W and 5 more authors..  (1993)  Phosphoglucomutase 1: a gene with two promoters and a duplicated first exon..  The Biochemical journal,  (1): [PMID:8257433]
6. Gururaj, Anupama A, Barnes, Christopher J CJ, Vadlamudi, Ratna K RK and Kumar, Rakesh R..  (2004)  Regulation of phosphoglucomutase 1 phosphorylation and activity by a signaling kinase..  Oncogene,  (21): [PMID:15378030]
7. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,  (18): [PMID:16710414]
8. Yu, Li-Rong LR and 5 more authors..  (2007)  Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra..  Journal of proteome research,  [PMID:17924679]
9. Stojkovic, Tanya T and 9 more authors..  (2009)  Muscle glycogenosis due to phosphoglucomutase 1 deficiency..  The New England journal of medicine,  (23): [PMID:19625727]
10. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,  (18): [PMID:19690332]
11. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,  (15): [PMID:21406692]
12. Timal, Sharita S and 18 more authors..  (2012)  Gene identification in the congenital disorders of glycosylation type I by whole-exome sequencing..  Human molecular genetics,  (1): [PMID:22492991]
13. Van Damme, Petra P and 15 more authors..  (2012)  N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB..  Proceedings of the National Academy of Sciences of the United States of America,  (31): [PMID:22814378]
14. Pérez, Belén B and 6 more authors..  (2013)  A novel congenital disorder of glycosylation type without central nervous system involvement caused by mutations in the phosphoglucomutase 1 gene..  Journal of inherited metabolic disease,  [PMID:22976764]
15. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,  (4): [PMID:23186163]
16. Tegtmeyer, Laura C LC and 49 more authors..  (2014)  Multiple phenotypes in phosphoglucomutase 1 deficiency..  The New England journal of medicine,  (6): [PMID:24499211]
17. Lee, Yingying Y, Stiers, Kyle M KM, Kain, Bailee N BN and Beamer, Lesa J LJ..  (2014)  Compromised catalysis and potential folding defects in in vitro studies of missense mutants associated with hereditary phosphoglucomutase 1 deficiency..  The Journal of biological chemistry,  (14): [PMID:25288802]
18. Stiers, Kyle M KM, Kain, Bailee N BN, Graham, Abigail C AC and Beamer, Lesa J LJ..  (2016)  Induced Structural Disorder as a Molecular Mechanism for Enzyme Dysfunction in Phosphoglucomutase 1 Deficiency..  Journal of molecular biology,  (24): [PMID:26972339]

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