| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab119000-10μl |
10μl |
现货  |
| |
| Ab119000-50μl |
50μL |
现货 |
| |
| Ab119000-100μl |
100μL |
现货 |
| |
| Ab119000-1ml |
1ml |
期货 |
|
| 产品名称 | Recombinant NUMB Antibody |
|---|---|
| 别名 | 重组Anti-NUMB 抗体 | NUMB 抗体 | 重组NUMB抗体 |
| 英文别名 | C14_5527 antibody | C14orf41 antibody | FLJ31314 antibody | H Numb antibody | H-Numb antibody | Numb antibody | Numb homolog (Drosophila) antibody | Numb homolog antibody | Numb protein homolog antibody | NUMB_HUMAN antibody | Protein numb homolog antibod |
| 规格或纯度 | ExactAb™, Validated, 重组, 0.5 mg/mL |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | Human NUMB |
| 应用 | WB,IHC |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 免疫原 | Recombinant protein of human NUMB (AA 413-651). |
| 阳性对照 | WB: MCF7, A549, HeLa, C6, NIH/3T3; IHC: Human lung cancer |
| 偶联 | Unconjugated |
| 抗体类型 | Primary antibody |
|---|---|
| 克隆类型 | 重组抗体 |
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Antigen affinity purified |
| 来源 | Cell supernatant |
| 物理外观 | Liquid |
| 储存缓冲液 | 50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4 |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.5 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Recombinant NUMB Antibody (Ab119000) - Western Blot
All lanes: Recombinant NUMB Antibody (Ab119000) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 72&74 kDa
Observed band size: 72&74 kDa
Recombinant NUMB Antibody (Ab119000) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant NUMB Antibody (Ab119000) . High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| 应用名称 | 稀释比例 |
|---|---|
| IHC | 1/50 - 1/100 |
| WB |
1/500-1/1000. Predicted molecular weight: 71 kDa.
|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F1011932 |
分析证书 | 24-10-12 | Ab119000 |
| ZJ23F0700727 |
分析证书 | 23-07-31 | Ab119000 |
| ZJ23F0700726 |
分析证书 | 23-07-31 | Ab119000 |
| 1. Sherrington, R R and 32 more authors.. (1995) Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease.. Nature, (29): [PMID:7596406] |
| 2. Salcini, A E AE and 8 more authors.. (1997) Binding specificity and in vivo targets of the EH domain, a novel protein-protein interaction module.. Genes & development, (1): [PMID:9303539] |
| 3. Verdi, J M JM and 6 more authors.. (1999) Distinct human NUMB isoforms regulate differentiation vs. proliferation in the neuronal lineage.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:10468633] |
| 4. Susini, L L and 11 more authors.. (2001) Siah-1 binds and regulates the function of Numb.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:11752454] |
| 5. Heilig, Roland R and 98 more authors.. (2003) The DNA sequence and analysis of human chromosome 14.. Nature, (6): [PMID:12508121] |
| 6. Rossé, Carine C, L'Hoste, Sébastien S, Offner, Nicolas N, Picard, André A and Camonis, Jacques J.. (2003) RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis.. The Journal of biological chemistry, (15): [PMID:12775724] |
| 7. Qin, Hanjuan H and 5 more authors.. (2004) A novel transmembrane protein recruits numb to the plasma membrane during asymmetric cell division.. The Journal of biological chemistry, (19): [PMID:14670962] |
| 8. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 9. Sorensen, Erika B EB and Conner, Sean D SD.. (2008) AAK1 regulates Numb function at an early step in clathrin-mediated endocytosis.. Traffic (Copenhagen, Denmark), [PMID:18657069] |
| 10. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 11. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 12. Haider, M M, Qiu, Q Q, Bani-Yaghoub, M M, Tsang, B K BK and Gruslin, A A.. (2011) Characterization and role of NUMB in the human extravillous trophopblast.. Placenta, [PMID:21486681] |
| 13. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
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