MUC1 Mouse mAb

    应用:
  • WB
功能和特点
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: C1
  • 偶联: Unconjugated
有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Ab116370-10μl
10μl 现货 Stock Image
Ab116370-50μl
50μL 现货 Stock Image
Ab116370-100μl
100μL 现货 Stock Image
Ab116370-1ml
1ml 期货 Stock Image

基本信息

产品名称 MUC1 Mouse mAb
别名 重组Anti-MUC1抗体 | 粘蛋白1单克隆抗体 | MUC1小鼠单克隆抗体 | MUC1小鼠单抗
英文别名 ADMCKD antibody | ADMCKD1 antibody | Breast carcinoma associated antigen DF3 antibody | Breast carcinoma-associated antigen DF3 antibody | CA 15-3 antibody | CA15 3 antibody | CA15 3 antigen antibody | CA15-3 antibody | CA15.3 antibody | Cancer antigen 15
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属 小鼠(Mouse)
特异性 MUC1
种属反应性 人(Human)
免疫原 Recombinant MUC1 expressed in E.coli (AA 1034~1151)
阳性对照 WB: Human milk.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 C1
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A + Protein G affinity purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

MUC1 Mouse mAb (Ab116370) - Western Blot
All lanes: MUC1 Mouse mAb (Ab116370) at 1/3000 dilution
Samples: Human milk
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 23,19 kDa
Exposure time: 10.0 s

关联靶点(人)

MUC1 Tchem 黏蛋白-1(Mucin-1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

安全和危险性(GHS)

质量标准

应用

应用名称 稀释比例
WB

0.01-2 µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到4个结果

批号(Lot Number) 证书类型 日期 货号
ZJ24F1011914 分析证书 24-10-12 Ab116370
ZJ23F1201971 分析证书 23-12-13 Ab116370
ZJ23F1201970 分析证书 23-12-13 Ab116370
ZJ23F1201969 分析证书 23-12-13 Ab116370

参考文献

1. Tsarfaty, I I and 9 more authors..  (1990)  Isolation and characterization of an expressed hypervariable gene coding for a breast-cancer-associated antigen..  Gene,  (14): [PMID:1688329]
2. Gendler, S J SJ and 8 more authors..  (1990)  Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin..  The Journal of biological chemistry,  (5): [PMID:1697589]
3. Hareuveni, M M and 9 more authors..  (1990)  A transcribed gene, containing a variable number of tandem repeats, codes for a human epithelial tumor antigen. cDNA cloning, expression of the transfected gene and over-expression in breast cancer tissue..  European journal of biochemistry,  (20): [PMID:2112460]
4. Lancaster, C A CA and 5 more authors..  (1990)  Structure and expression of the human polymorphic epithelial mucin gene: an expressed VNTR unit..  Biochemical and biophysical research communications,  (31): [PMID:2268309]
5. Ligtenberg, M J MJ, Vos, H L HL, Gennissen, A M AM and Hilkens, J J..  (1990)  Episialin, a carcinoma-associated mucin, is generated by a polymorphic gene encoding splice variants with alternative amino termini..  The Journal of biological chemistry,  (5): [PMID:2318825]
6. Wreschner, D H DH and 9 more authors..  (1990)  Human epithelial tumor antigen cDNA sequences. Differential splicing may generate multiple protein forms..  European journal of biochemistry,  (20): [PMID:2351132]
7. Lan, M S MS, Batra, S K SK, Qi, W N WN, Metzgar, R S RS and Hollingsworth, M A MA..  (1990)  Cloning and sequencing of a human pancreatic tumor mucin cDNA..  The Journal of biological chemistry,  (5): [PMID:2394722]
8. Abe, M M, Siddiqui, J J and Kufe, D D..  (1989)  Sequence analysis of the 5' region of the human DF3 breast carcinoma-associated antigen gene..  Biochemical and biophysical research communications,  (15): [PMID:2597151]
9. Gendler, S S, Taylor-Papadimitriou, J J, Duhig, T T, Rothbard, J J and Burchell, J J..  (1988)  A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats..  The Journal of biological chemistry,  (15): [PMID:3417635]
10. Pandey, P P, Kharbanda, S S and Kufe, D D..  (1995)  Association of the DF3/MUC1 breast cancer antigen with Grb2 and the Sos/Ras exchange protein..  Cancer research,  (15): [PMID:7664271]
11. Zrihan-Licht, S S and 7 more authors..  (1994)  Characterization and molecular cloning of a novel MUC1 protein, devoid of tandem repeats, expressed in human breast cancer tissue..  European journal of biochemistry,  (1): [PMID:7925397]
12. Zrihan-Licht, S S, Baruch, A A, Elroy-Stein, O O, Keydar, I I and Wreschner, D H DH..  (1994)  Tyrosine phosphorylation of the MUC1 breast cancer membrane proteins. Cytokine receptor-like molecules..  FEBS letters,  (12): [PMID:7988707]
13. Yu, C J CJ and 8 more authors.. Mucin mRNA expression in lung adenocarcinoma cell lines and tissues..  Oncology,  [PMID:8604237]
14. Weiss, M M, Baruch, A A, Keydar, I I and Wreschner, D H DH..  (1996)  Preoperative diagnosis of thyroid papillary carcinoma by reverse transcriptase polymerase chain reaction of the MUC1 gene..  International journal of cancer,  (28): [PMID:8608966]
15. Yamamoto, M M, Bharti, A A, Li, Y Y and Kufe, D D..  (1997)  Interaction of the DF3/MUC1 breast carcinoma-associated antigen and beta-catenin in cell adhesion..  The Journal of biological chemistry,  (9): [PMID:9139698]
16. Oosterkamp, H M HM, Scheiner, L L, Stefanova, M C MC, Lloyd, K O KO and Finstad, C L CL..  (1997)  Comparison of MUC-1 mucin expression in epithelial and non-epithelial cancer cell lines and demonstration of a new short variant form (MUC-1/Z)..  International journal of cancer,  (3): [PMID:9212228]
17. Müller, S S and 5 more authors..  (1997)  Localization of O-glycosylation sites on glycopeptide fragments from lactation-associated MUC1. All putative sites within the tandem repeat are glycosylation targets in vivo..  The Journal of biological chemistry,  (3): [PMID:9312074]
18. Li, Y Y, Bharti, A A, Chen, D D, Gong, J J and Kufe, D D..  (1998)  Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin..  Molecular and cellular biology,  [PMID:9819408]
19. Baruch, A A and 10 more authors..  (1999)  The breast cancer-associated MUC1 gene generates both a receptor and its cognate binding protein..  Cancer research,  (1): [PMID:10197628]
20. Müller, S S and 5 more authors..  (1999)  High density O-glycosylation on tandem repeat peptide from secretory MUC1 of T47D breast cancer cells..  The Journal of biological chemistry,  (25): [PMID:10373415]
21. Li, Y Y, Kuwahara, H H, Ren, J J, Wen, G G and Kufe, D D..  (2001)  The c-Src tyrosine kinase regulates signaling of the human DF3/MUC1 carcinoma-associated antigen with GSK3 beta and beta-catenin..  The Journal of biological chemistry,  (2): [PMID:11152665]
22. Parry, S S and 5 more authors..  (2001)  Identification of MUC1 proteolytic cleavage sites in vivo..  Biochemical and biophysical research communications,  (11): [PMID:11341784]
23. Engelmann, K K, Baldus, S E SE and Hanisch, F G FG..  (2001)  Identification and topology of variant sequences within individual repeat domains of the human epithelial tumor mucin MUC1..  The Journal of biological chemistry,  (27): [PMID:11350974]
24. Ren, Jian J, Li, Yongqing Y and Kufe, Donald D..  (2002)  Protein kinase C delta regulates function of the DF3/MUC1 carcinoma antigen in beta-catenin signaling..  The Journal of biological chemistry,  (17): [PMID:11877440]
25. Thathiah, Amantha A, Blobel, Carl P CP and Carson, Daniel D DD..  (2003)  Tumor necrosis factor-alpha converting enzyme/ADAM 17 mediates MUC1 shedding..  The Journal of biological chemistry,  (31): [PMID:12441351]
26. Li, Yongqing Y and 7 more authors..  (2003)  Heregulin targets gamma-catenin to the nucleolus by a mechanism dependent on the DF3/MUC1 oncoprotein..  Molecular cancer research : MCR,  [PMID:12939402]
27. Huang, Lei L and 5 more authors.. MUC1 cytoplasmic domain coactivates Wnt target gene transcription and confers transformation..  Cancer biology & therapy,  [PMID:14688481]
28. Kinlough, Carol L CL, Poland, Paul A PA, Bruns, James B JB, Harkleroad, Keri L KL and Hughey, Rebecca P RP..  (2004)  MUC1 membrane trafficking is modulated by multiple interactions..  The Journal of biological chemistry,  (17): [PMID:15471854]
29. Mukherjee, P P, Tinder, T L TL, Basu, G D GD and Gendler, S J SJ..  (2005)  MUC1 (CD227) interacts with lck tyrosine kinase in Jurkat lymphoma cells and normal T cells..  Journal of leukocyte biology,  [PMID:15513966]
30. Levitin, Fiana F and 19 more authors..  (2005)  A novel protein derived from the MUC1 gene by alternative splicing and frameshifting..  The Journal of biological chemistry,  (18): [PMID:15623537]
31. Wei, Xiaolong X, Xu, Hai H and Kufe, Donald D..  (2005)  Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response..  Cancer cell,  [PMID:15710329]
32. Zhang, Li-Xin LX, Li, Chun-Hai CH, Sun, Li-Ya LY, Wang, Miao M and Lu, Hao-Jun HJ..  (2003)  [Soluble expression of peptide containing MUC1/Y-specific epitope in Escherichia coli and preparation of the antibody]..  Sheng wu gong cheng xue bao = Chinese journal of biotechnology,  [PMID:15969018]
33. Engelmann, Katja K and 6 more authors..  (2005)  Transmembrane and secreted MUC1 probes show trafficking-dependent changes in O-glycan core profiles..  Glycobiology,  [PMID:15972891]
34. Levitin, Fiana F and 8 more authors..  (2005)  The MUC1 SEA module is a self-cleaving domain..  The Journal of biological chemistry,  (30): [PMID:15987679]
35. Huang, Lei L and 5 more authors..  (2005)  MUC1 oncoprotein blocks glycogen synthase kinase 3beta-mediated phosphorylation and degradation of beta-catenin..  Cancer research,  (15): [PMID:16288032]
36. Wei, Xiaolong X, Xu, Hai H and Kufe, Donald D..  (2006)  MUC1 oncoprotein stabilizes and activates estrogen receptor alpha..  Molecular cell,  (20): [PMID:16427018]
37. Parry, Simon S and 6 more authors..  (2006)  N-Glycosylation of the MUC1 mucin in epithelial cells and secretions..  Glycobiology,  [PMID:16585136]
38. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,  (18): [PMID:16710414]
39. Pochampalli, M R MR, el Bejjani, R M RM and Schroeder, J A JA..  (2007)  MUC1 is a novel regulator of ErbB1 receptor trafficking..  Oncogene,  (15): [PMID:16983337]
40. Wei, Xiaolong X, Xu, Hai H and Kufe, Donald D..  (2007)  Human mucin 1 oncoprotein represses transcription of the p53 tumor suppressor gene..  Cancer research,  (15): [PMID:17308127]
41. Lillehoj, Erik P EP, Lu, Wenju W, Kiser, Timothy T, Goldblum, Simeon E SE and Kim, K Chul KC..  (2007)  MUC1 inhibits cell proliferation by a beta-catenin-dependent mechanism..  Biochimica et biophysica acta,  [PMID:17524503]
42. Singh, Pankaj K PK and 7 more authors..  (2007)  Platelet-derived growth factor receptor beta-mediated phosphorylation of MUC1 enhances invasiveness in pancreatic adenocarcinoma cells..  Cancer research,  (1): [PMID:17545600]
43. Duffy, Michael J MJ, Evoy, Denis D and McDermott, Enda W EW..  (2010)  CA 15-3: uses and limitation as a biomarker for breast cancer..  Clinica chimica acta; international journal of clinical chemistry,  (14): [PMID:20816948]
44. Zhang, Lixin L, Vlad, Anda A, Milcarek, Christine C and Finn, Olivera J OJ..  (2013)  Human mucin MUC1 RNA undergoes different types of alternative splicing resulting in multiple isoforms..  Cancer immunology, immunotherapy : CII,  [PMID:22941036]
45. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,  (4): [PMID:23186163]
46. Kirby, Andrew A and 42 more authors..  (2013)  Mutations causing medullary cystic kidney disease type 1 lie in a large VNTR in MUC1 missed by massively parallel sequencing..  Nature genetics,  [PMID:23396133]
47. Li, Y Y and 7 more authors..  (2001)  The epidermal growth factor receptor regulates interaction of the human DF3/MUC1 carcinoma antigen with c-Src and beta-catenin..  The Journal of biological chemistry,  (21): [PMID:11483589]

溶液计算器