IL-10 Mouse mAb

    应用:
  • Capture Antibody
  • CLIA
  • ELISA
  • FIA
  • FLFIA
  • GICA
  • LF
功能和特点
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: 3G12-7
  • 偶联: Unconjugated
有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Ab109811-10μg
10μg 现货 Stock Image
Ab109811-50μg
50μg 现货 Stock Image
Ab109811-100μg
100μg 现货 Stock Image
Ab109811-1mg
1mg 期货 Stock Image

基本信息

产品名称 IL-10 Mouse mAb
别名 Anti-IL-10抗体 | 抗IL-10 | 抗IL-10抗体 | IL-10 小鼠单克隆抗体 | IL-10 小鼠单抗
英文别名 CSIF | CSIFMGC126450 | Cytokine synthesis inhibitory factor | GVHDS | IL10 | IL-10 | IL10A | IL-10MGC126451 | interleukin 10 | interleukin-10 | TGIF | CSIF antibody | Cytokine synthesis inhibitory factor antibody | GVHDS antibody | IL 10 antibody | IL-10
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属 小鼠(Mouse)
特异性 IL10
种属反应性 人(Human)
免疫原 Recombinant Human IL-10 Protein
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 3G12-7
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 25.5 kDa (Light Chain) & 49.7 kDa (Heavy Chain), under reducing conditions; 170.2 kDa, under non-reducing conditions.
纯化方法 Affinity chromatography
配对抗体 [Ab109817] IL-10 Mouse mAb as Detection antibody in ELISA, CLIA, LF, GICA, FIA, FACS
物理外观 Liquid
储存缓冲液 10mM PBS, pH7.4
防腐剂 No
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 37°C 下短期保存(7 天) 4°C 下短期保存(30 天)。在 -20±5°C 下长期储存(36 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

Sandwich ELISA - IL-10 protein standard curve
Human IL-10 protein was serially diluted 2-fold and captured by IL-10 Mouse mAb (Ab109811) coated on a Clear Polystyrene Microplate (W291037). IL-10 Mouse mAb (Ab109817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).

Relevance of detection results between aladdin Antibody Pair (Ab109811 & Ab109817) in CLIA
·Capture antibody: IL-10 Mouse mAb (Ab109811)
·Detection antibody: IL-10 Mouse mAb (Ab109817)

Binding ELISA - IL-10 Mouse mAb (Ab109811)
Immobilized Recombinant Human IL-10 Protein at 1.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC₅₀ of 31.66 ng/mL.

关联靶点(人)

IL10 Tbio 白细胞介素-10(Interleukin-10) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

安全和危险性(GHS)

质量标准

应用

应用名称 稀释比例
CLIA

20µg/2mg magnetic beads

FLFIA

Fluorescent beads/Antibody =10/1

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到1个结果

批号(Lot Number) 证书类型 日期 货号
ZJ24F1011945 分析证书 24-10-12 Ab109811

参考文献

1. Vieira, P P and 9 more authors..  (1991)  Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI..  Proceedings of the National Academy of Sciences of the United States of America,  (15): [PMID:1847510]
2. de Waal Malefyt, R R, Abrams, J J, Bennett, B B, Figdor, C G CG and de Vries, J E JE..  (1991)  Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes..  The Journal of experimental medicine,  (1): [PMID:1940799]
3. Walter, M R MR and Nagabhushan, T L TL..  (1995)  Crystal structure of interleukin 10 reveals an interferon gamma-like fold..  Biochemistry,  (26): [PMID:7547951]
4. Zdanov, A A and 5 more authors..  (1995)  Crystal structure of interleukin-10 reveals the functional dimer with an unexpected topological similarity to interferon gamma..  Structure (London, England : 1993),  (15): [PMID:8590020]
5. Gesser, B B and 8 more authors..  (1997)  Identification of functional domains on human interleukin 10..  Proceedings of the National Academy of Sciences of the United States of America,  (23): [PMID:9405662]
6. Josephson, K K, Logsdon, N J NJ and Walter, M R MR..  (2001)  Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site..  Immunity,  [PMID:11485736]
7. van der Linde, K K and 6 more authors..  (2003)  A Gly15Arg mutation in the interleukin-10 gene reduces secretion of interleukin-10 in Crohn disease..  Scandinavian journal of gastroenterology,  [PMID:12825869]
8. Lin, Ming-Tseh MT and 6 more authors..  (2003)  Relation of an interleukin-10 promoter polymorphism to graft-versus-host disease and survival after hematopoietic-cell transplantation..  The New England journal of medicine,  (4): [PMID:14657427]
9. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,  [PMID:15340161]
10. Yoon, Sung Il SI, Jones, Brandi C BC, Logsdon, Naomi J NJ and Walter, Mark R MR..  (2005)  Same structure, different function crystal structure of the Epstein-Barr virus IL-10 bound to the soluble IL-10R1 chain..  Structure (London, England : 1993),  [PMID:15837194]
11. Yoon, Sung Il SI, Logsdon, Naomi J NJ, Sheikh, Faruk F, Donnelly, Raymond P RP and Walter, Mark R MR..  (2006)  Conformational changes mediate interleukin-10 receptor 2 (IL-10R2) binding to IL-10 and assembly of the signaling complex..  The Journal of biological chemistry,  (17): [PMID:16982608]
12. El Kasmi, Karim C KC and 9 more authors..  (2007)  Cutting edge: A transcriptional repressor and corepressor induced by the STAT3-regulated anti-inflammatory signaling pathway..  Journal of immunology (Baltimore, Md. : 1950),  (1): [PMID:18025162]
13. Windsor, W T WT and 9 more authors..  (1993)  Disulfide bond assignments and secondary structure analysis of human and murine interleukin 10..  Biochemistry,  (31): [PMID:8364028]
14. Zdanov, A A, Schalk-Hihi, C C and Wlodawer, A A..  (1996)  Crystal structure of human interleukin-10 at 1.6 A resolution and a model of a complex with its soluble receptor..  Protein science : a publication of the Protein Society,  [PMID:8897595]

溶液计算器