FAM120A/OSSA Mouse mAb

无载体

已验证

ExactAb™

0.5 mg/mL

应用:

IF/ICC
IHC
WB

功能和特点

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: F01S803
偶联: Unconjugated

有货

库存信息

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货号 (SKU)	包装规格	是否现货
Ab102814-10μl	10μl	现货
Ab102814-50μl	50μL	现货
Ab102814-100μl	100μL	期货
Ab102814-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: FAM120A

Accession#: Q9NZB2 (1) FAM120A (1) Gene ID: 23196 (1) OSSA (1)

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基本信息

产品名称	FAM120A/OSSA Mouse mAb
别名	家族相似性蛋白120A抗体 \| FAM120A单克隆抗体
英文别名	C9orf10 antibody \| Chromosome 9 open reading frame 10 antibody \| Constitutive coactivator of PPAR gamma like protein 1 antibody \| Constitutive coactivator of PPAR-gamma-like protein 1 antibody \| DNA polymerase transactivated protein5 antibody \| DNA polyme
规格或纯度	ExactAb™, 已验证, 无载体, 0.5 mg/mL
宿主种属	小鼠(Mouse)
特异性	FAM120A
种属反应性	人(Human)
免疫原	Recombinant fragment within Human FAM120A/OSSA (internal sequence). The exact sequence is proprietary.
阳性对照	WB: MCF7 and SK-N-SH cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 30% Glycerol, 0.01% Sodium azide, pH 7.3
防腐剂	0.01% Sodium azide
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（12个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

图片

Western Blot: FAM120A/OSSA Mouse mAb (Ab102814)

FAM120A/OSSA Mouse mAb (Ab102814) - Western Blot
All lanes: FAM120A/OSSA Mouse mAb (Ab102814) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution
Predicted band size: 121 kDa
Observed band size: 145 kDa
Exposure time: 100.0 s

IHC: FAM120A/OSSA Mouse mAb (Ab102814)

FAM120A/OSSA Mouse mAb (Ab102814) - IHC
Immunohistochemical analysis of FAM120A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FAM120A/OSSA Mouse mAb (Ab102814) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: FAM120A/OSSA Mouse mAb (Ab102814)

FAM120A/OSSA Mouse mAb (Ab102814) - IF/ICC
Immunofluorescent analysis of FAM120A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with FAM120A/OSSA Mouse mAb (Ab102814) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).