UBA1 Antibody

    应用:
  • IHC
  • WB
功能和特点
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab100767-10μl
10μl 现货 Stock Image
Ab100767-50μl
50μL 现货 Stock Image
Ab100767-100μl
100μL 期货 Stock Image
Ab100767-1ml
1ml 期货 Stock Image

基本信息

产品名称 UBA1 Antibody
别名 泛素激活酶1抗体 | UBA1抗体
英文别名 EC 6.2.1.45 | Protein A1S9 | Ubiquitin-activating enzyme E1 | A1S9 antibody | A1S9 protein antibody | A1S9T and BN75 temperature sensitivity complementing antibody | A1S9T antibody | A1ST antibody | AMCX1 antibody | CFAP124 antibody | CTD-2522E6.1 antibod
规格或纯度 ExactAb™, Validated, Carrier Free, 0.5 mg/mL
宿主种属 兔(Rabbit)
特异性 UBA1
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 A KLH conjugated synthetic peptide from the N-terminal region of human UBA1 (AA 1-30)
阳性对照 WB: K562, HeLa, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue.
偶联 Unconjugated

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Protein A purified + Peptide affinity purified
物理外观 Liquid
储存缓冲液 10mM PBS, 0.09% Sodium azide, pH 7.4
防腐剂 0.09% Sodium azide
浓度 0.5 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

UBA1 Antibody (Ab100767) Western Blot
All lanes: UBA1 Antibody (Ab100767) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution

Predicted band size: 118 kDa
Observed band size: 116 kDa
Exposure time: 6.5 s

UBA1 Antibody (Ab100767) - IHC
UBA1 Antibody (Ab100767) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UBA1 Antibody (Ab100767) for immunohistochemistry. Clinical relevance has not been evaluated.

关联靶点(人)

UBA1 Tbio 泛素类修饰激活酶 1(Ubiquitin-like modifier-activating enzyme 1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

安全和危险性(GHS)

质量标准

应用

应用名称 稀释比例
WB

1/1000

IHC

1/100-1/500

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到2个结果

批号(Lot Number) 证书类型 日期 货号
ZJ24F0303276 分析证书 24-03-14 Ab100767
ZJ24F0303275 分析证书 24-03-14 Ab100767

参考文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al..  (2017)  Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors..  Bioorg Med Chem,  25  (4): (1320-1328).  [PMID:28038940]
3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
4. Wang RE, Hunt CR, Chen J, Taylor JS..  (2011)  Biotinylated quercetin as an intrinsic photoaffinity proteomics probe for the identification of quercetin target proteins..  Bioorg Med Chem,  19  (16): (4710-4720).  [PMID:21798748] [10.1016/j.bmc.2011.07.005]
5. Wu KJ, Zhong HJ, Li G, Liu C, Wang HD, Ma DL, Leung CH..  (2018)  Structure-based identification of a NEDD8-activating enzyme inhibitor via drug repurposing..  Eur J Med Chem,  143  (1021-1027).  [PMID:29232579] [10.1016/j.ejmech.2017.11.101]
6. Itoh Y, Suzuki M..  (2018)  Design, synthesis, and biological evaluation of novel ubiquitin-activating enzyme inhibitors..  Bioorg Med Chem Lett,  28  (16): (2723-2727).  [PMID:29548576] [10.1016/j.bmcl.2018.03.004]
7. Schwartz, A L AL, Trausch, J S JS, Ciechanover, A A, Slot, J W JW and Geuze, H H..  (1992)  Immunoelectron microscopic localization of the ubiquitin-activating enzyme E1 in HepG2 cells..  Proceedings of the National Academy of Sciences of the United States of America,  (15): [PMID:1376922]
8. Ayusawa, D D and 7 more authors..  (1992)  Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1..  Cell structure and function,  [PMID:1606621]
9.  (1991)  Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1..  Proceedings of the National Academy of Sciences of the United States of America,  (15): [PMID:1871145]
10. Handley, P M PM, Mueckler, M M, Siegel, N R NR, Ciechanover, A A and Schwartz, A L AL..  (1991)  Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1..  Proceedings of the National Academy of Sciences of the United States of America,  (1): [PMID:1986373]
11. Zacksenhaus, E E and Sheinin, R R..  (1990)  Molecular cloning, primary structure and expression of the human X linked A1S9 gene cDNA which complements the ts A1S9 mouse L cell defect in DNA replication..  The EMBO journal,  [PMID:2390975]
12. Handley-Gearhart, P M PM, Stephen, A G AG, Trausch-Azar, J S JS, Ciechanover, A A and Schwartz, A L AL..  (1994)  Human ubiquitin-activating enzyme, E1. Indication of potential nuclear and cytoplasmic subpopulations using epitope-tagged cDNA constructs..  The Journal of biological chemistry,  (30): [PMID:7528747]
13. Rush, John J and 9 more authors..  (2005)  Immunoaffinity profiling of tyrosine phosphorylation in cancer cells..  Nature biotechnology,  [PMID:15592455]
14. Ross, Mark T MT and 281 more authors..  (2005)  The DNA sequence of the human X chromosome..  Nature,  (17): [PMID:15772651]
15. Giannakopoulos, Nadia V NV and 9 more authors..  (2005)  Proteomic identification of proteins conjugated to ISG15 in mouse and human cells..  Biochemical and biophysical research communications,  (21): [PMID:16139798]
16. Allen, Elizabeth E and 6 more authors..  (2005)  Gigaxonin-controlled degradation of MAP1B light chain is critical to neuronal survival..  Nature,  (10): [PMID:16227972]
17. Ramser, Juliane J and 11 more authors..  (2008)  Rare missense and synonymous variants in UBE1 are associated with X-linked infantile spinal muscular atrophy..  American journal of human genetics,  [PMID:18179898]
18. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,  (5): [PMID:18669648]
19. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,  (18): [PMID:19690332]
20. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,  (15): [PMID:21406692]
21. Moudry, Pavel P and 6 more authors..  (2012)  Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage..  Cell cycle (Georgetown, Tex.),  (15): [PMID:22456334]
22. Van Damme, Petra P and 15 more authors..  (2012)  N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB..  Proceedings of the National Academy of Sciences of the United States of America,  (31): [PMID:22814378]
23. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,  (4): [PMID:23186163]
24. Dlamini, Nomazulu N and 12 more authors..  (2013)  Clinical and neuropathological features of X-linked spinal muscular atrophy (SMAX2) associated with a novel mutation in the UBA1 gene..  Neuromuscular disorders : NMD,  [PMID:23518311]
25. and Xie, Shu-Tao ST..  (2014)  Expression, purification, and crystal structure of N-terminal domains of human ubiquitin-activating enzyme (E1)..  Bioscience, biotechnology, and biochemistry,  [PMID:25209502]
26. Beck, David B and 61 more authors..  (2020)  Somatic Mutations in UBA1 and Severe Adult-Onset Autoinflammatory Disease..  The New England journal of medicine,  (31): [PMID:33108101]
27. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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