| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab098695-10μl |
10μl |
期货  |
| |
| Ab098695-50μl |
50μL |
现货 |
| |
| Ab098695-100μl |
100μL |
现货 |
| |
| Ab098695-1ml |
1ml |
期货 |
|
| 产品名称 | Cytochrome C Mouse mAb |
|---|---|
| 别名 | 细胞色素C抗体 | 细胞色素C单克隆抗体 | 细胞色素C重组单克隆抗体 | Cytochrome C抗体 | Cytochrome C单克隆抗体 | Cytochrome C重组单克隆抗体 | CYCS抗体 | CYCS单克隆抗体 | CYCS重组单克隆抗体 |
| 英文别名 | CYC antibody | CYC_HUMAN antibody | CYCS antibody | Cytochrome c antibody | Cytochrome c somatic antibody | HCS antibody | THC4 antibody | Monoclonal Antibody to Cytochrome C |
| 规格或纯度 | 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA |
| 生化机理 | Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | CYCS |
| 种属反应性 | 人(Human),原鸡属(Gallus),大鼠(Rat),小鼠(Mouse),山羊(Goat),狗(Dog),猪(Pig),豚鼠(Guinea pig) |
| 免疫原 | Recombinant CYCS expressed in E.coli (AA 5-100) |
| 阳性对照 | WB: HEK293, Jurkat, HeLa, Hep G2, A549 and LNCaP cell lysates. IHC: Human cerebellum, cardiac muscle, small intestine, lymph node tissues. ICC/IF: Hep G2, HeLa cells. |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | C2 |
| Format | Whole IgG |
| 亚型 | Mouse IgG2a |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A + Protein G purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4 |
| 防腐剂 | 0.05% Proclin 300 |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融 |
| 分子类型 | 抗体 |
Cytochrome C Mouse mAb (Ab098695) - Western Blot
All lanes: Cytochrome C Mouse mAb (Ab098695) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 16 kDa
Exposure time: 7.9 s
Cytochrome C Mouse mAb (Ab098695) - IF/ICC
FITC staining on IF
Sample: Human Hep G2 cell
Primary Ab: 20 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 5 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IF/ICC
FITC staining on IF
Sample: Human HeLa cell
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC
DAB staining on IHC
Sample: Human Cerebellum Tissue
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC
DAB staining on IHC
Sample: Human Lymph node Tissue
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC |
5-30 µg/mL
|
| IHC | 5-30 µg/mL |
| WB |
0.01-2 µg/mL
|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F1011798 |
分析证书 | 24-10-11 | Ab098695 |
| ZJ23F1101824 |
分析证书 | 23-11-27 | Ab098695 |
| ZJ23F1101821 |
分析证书 | 23-11-27 | Ab098695 |
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| 10. Chu, Heng-Cheng HC and 5 more authors.. (2005) Effects of minocycline on Fas-mediated fulminant hepatitis in mice.. British journal of pharmacology, [PMID:15665864] |
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| 13. San Francisco, Brian B, Bretsnyder, Eric C EC and Kranz, Robert G RG.. (2013) Human mitochondrial holocytochrome c synthase's heme binding, maturation determinants, and complex formation with cytochrome c.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:23150584] |
| 14. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 15. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
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