基本信息

产品名称	Coatomer subunit delta/COPD Mouse mAb
别名	古菌素1(ARCN1)单克隆抗体
英文别名	Archain 1 antibody \| Archain antibody \| Archain vesicle transport protein 1 antibody \| Arcn1 antibody \| Coatomer delta subunit antibody \| Coatomer protein complex, subunit delta antibody \| Coatomer protein delta COP antibody \| Coatomer subunit delta antib
规格或纯度	ExactAb™, 已验证, 见COA
生化机理	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
宿主种属	小鼠(Mouse)
特异性	ARCN1
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),狗(Dog),猴(Monkey)
免疫原	Recombinant full length protein of human Coatomer subunit delta/COPD.
阳性对照	WB: Hep G2, HeLa and HEK293 cell lysates. IHC: Human colon adenocarcinoma, pancreas, endometrium adenocarcinoma, prostate carcinoma tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY ARCN1. Flow Cytometry: HEK293T cells transfected with
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 1% BSA, 0.02% Sodium azide, pH 7.3
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 运输。发货后，分装并储存于 -20°C 或 -80°C。避免反复冻融循环。
分子类型	抗体

图片

Western Blot: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - Western Blot
All lanes: Coatomer subunit delta/COPD Mouse mAb (Ab097079) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 60 kDa
Exposure time: 54.2 s

IHC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human colon tissue using Coatomer subunit delta/COPD Mouse mAb (Ab097079). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using Coatomer subunit delta/COPD Mouse mAb (Ab097079). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human endometrium tissue using Coatomer subunit delta/COPD Mouse mAb (Ab097079). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human prostate tissue using Coatomer subunit delta/COPD Mouse mAb (Ab097079). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IF/ICC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IF/ICC
Coatomer subunit delta/COPD Mouse mAb (Ab097079) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARCN1 .

Flow Cytometry: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by Coatomer subunit delta/COPD Mouse mAb (Ab097079), and then analyzed by flow cytometry.