| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab095561-10μl |
10μl |
现货  |
| |
| Ab095561-50μl |
50μL |
现货 |
| |
| Ab095561-100μl |
100μL |
期货 |
| |
| Ab095561-1ml |
1ml |
期货 |
|
| 产品名称 | CDA Mouse mAb |
|---|---|
| 别名 | 胞苷脱氨酶(CDA)单克隆抗体 |
| 英文别名 | DD antibody | Cytidine aminohydrolaseantibody | Cytidine deaminase antibody | EC 3.5.4.5 antibody | CDA | CDD |
| 规格或纯度 | 无载体, ExactAb™, 已验证, 1.0 mg/mL |
| 生化机理 | 这种酶可清除外源性和内源性胞苷和 2'- 脱氧胞苷，用于合成 UMP。 |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | CDA |
| 种属反应性 | 人(Human) |
| 免疫原 | Recombinant protein of human CDA (AA 1-146) |
| 阳性对照 | WB: HeLa, SK-OV-3 lysates. IHC: Human spleen tissue. IP: SK-OV-3 lysates. |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 150mM PBS, 0.09% Sodium azide, pH 7.4 |
| 防腐剂 | 0.09% Sodium azide |
| 浓度 | 1.0 mg/mL |
| 储存温度 | -80℃储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 2-8 ℃ 下保存（3 个月）。在 -20 ℃ 下保存（12 个月）。在 -80 ℃ 下保存（36 个月）。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
CDA Mouse mAb (Ab095561) - Western Blot
All lanes: CDA Mouse mAb (Ab095561) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 15.0 s
CDA Mouse mAb (Ab095561) - IHC
IHC-P was performed using sections of the formalin-fixed paraffin-embedded spleen tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with CDA Mouse mAb (Ab095561) at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cells in red pulp are positively stained.
CDA Mouse mAb (Ab095561) - IP
Immunoprecipitation was performed by incubation of 2.5 µg CDA Mouse mAb (Ab095561) with SK-OV-3 lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. CDA Mouse mAb (Ab095561) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Lane 1: SK-OV-3 lysate
Lane 2: CDA immunoprecipitated from SK-OV-3 lysate by CDA Mouse mAb (Ab095561)
Lane 3: The same as Lane 2 but Mouse IgG was used as IgG isotype control antibody
Result: CDA Mouse mAb (Ab095561) can immunoprecipitate CDA.
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 应用名称 | 稀释比例 |
|---|---|
| WB |
1/1000
|
| IP | 1/100 |
| IHC | 1/400 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F1114177 |
分析证书 | 24-11-22 | Ab095561 |
| ZJ24F1114176 |
分析证书 | 24-11-22 | Ab095561 |
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