CD276 Antibody

    应用:
  • IHC
  • WB
功能和特点
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab094381-10μl
10μl 现货 Stock Image
Ab094381-50μl
50μL 现货 Stock Image
Ab094381-100μl
100μL 现货 Stock Image
Ab094381-1ml
1ml 期货 Stock Image

基本信息

产品名称 CD276 Antibody
别名 CD276分子多克隆抗体 | CD276 抗体
英文别名 4Ig B7 H3 antibody | 4Ig-B7-H3 antibody | AU016588 antibody | B7 H3 antibody | B7 homolog 3 antibody | B7-H3 antibody | B7H3 antibody | B7RP-2 antibody | CD_antigen=CD276 antibody | CD276 antibody | CD276 antigen antibody | CD276 molecule antibody | CD276
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属 兔(Rabbit)
特异性 CD276
种属反应性 人(Human)
免疫原 Recombinant CD276 expressed in E.coli (AA 47-219)
阳性对照 WB: HeLa, LNCAP, HEK293 and MCF7 cell lysates. IHC: Human Liver cancer tissue, Human Kidney tissue. Flow: CHO-K1 transfected with Human CD276.
偶联 Unconjugated

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Antigen affinity purified and Protein A purified
物理外观 Liquid
储存缓冲液 10 mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

CD276 Antibody (Ab094381) - Western Blot
All lanes: CD276 Antibody (Ab094381) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 90 kDa
Observed band size: 92 kDa
Exposure time: 71.7 s

CD276 Antibody (Ab094381) - Flow Cytometry
CHO-K1 transfected with human CD276 (Stable transfection) was stained with CD276 Antibody (Ab094381) (filled red histogram) or Isotype control antibody (filled blue histogram), followed by Cy5-conjugated Anti-rabbit IgG Secondary Antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

CD276 Antibody (Ab094381) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 μg/mL CD276 Antibody (Ab094381)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

CD276 Antibody (Ab094381) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 μg/mL CD276 Antibody (Ab094381)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

关联靶点(人)

CD276 Tbio CD276抗原(CD276 antigen) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

安全和危险性(GHS)

质量标准

应用

应用名称 稀释比例
IHC

5-30 µg/mL

WB

0.01-3 µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到2个结果

批号(Lot Number) 证书类型 日期 货号
ZJ24F0911349 分析证书 24-09-27 Ab094381
ZJ23F0901087 分析证书 23-09-18 Ab094381

参考文献

1. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al..  (2003)  The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment..  Genome Res,  13  (10): (2265-70).  [PMID:12975309]
2. Loo D, Alderson RF, Chen FZ, Huang L, Zhang W, Gorlatov S, Burke S, Ciccarone V, Li H, Yang Y et al..  (2012)  Development of an Fc-enhanced anti-B7-H3 monoclonal antibody with potent antitumor activity..  Clin Cancer Res,  18  (14): (3834-45).  [PMID:22615450]
3. Chapoval AI, Ni J, Lau JS, Wilcox RA, Flies DB, Liu D, Dong H, Sica GL, Zhu G, Tamada K et al..  (2001)  B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production..  Nat Immunol,  (3): (269-74).  [PMID:11224528]
4. Castriconi R, Dondero A, Augugliaro R, Cantoni C, Carnemolla B, Sementa AR, Negri F, Conte R, Corrias MV, Moretta L et al..  (2004)  Identification of 4Ig-B7-H3 as a neuroblastoma-associated molecule that exerts a protective role from an NK cell-mediated lysis..  Proc Natl Acad Sci USA,  101  (34): (12640-5).  [PMID:15314238]
5. Ling, Vincent V and 6 more authors..  (2003)  Duplication of primate and rodent B7-H3 immunoglobulin V- and C-like domains: divergent history of functional redundancy and exon loss..  Genomics,  [PMID:12906861]
6. Steinberger, Peter P and 9 more authors..  (2004)  Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:14764704]
7. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,  [PMID:15340161]
8. Wang, Liqing L and 7 more authors..  (2005)  B7-H3 promotes acute and chronic allograft rejection..  European journal of immunology,  [PMID:15682454]
9. Kim, Jean J and 8 more authors..  (2005)  Constitutive and inducible expression of b7 family of ligands by human airway epithelial cells..  American journal of respiratory cell and molecular biology,  [PMID:15961727]
10. Petroff, Margaret G MG, Kharatyan, Elza E, Torry, Donald S DS and Holets, Lesya L..  (2005)  The immunomodulatory proteins B7-DC, B7-H2, and B7-H3 are differentially expressed across gestation in the human placenta..  The American journal of pathology,  [PMID:16049332]
11. Zhang, Guangbo G, Dong, Qiuming Q, Xu, Ying Y, Yu, Gehua G and Zhang, Xueguang X..  (2005)  B7-H3: another molecule marker for Mo-DCs?.  Cellular & molecular immunology,  [PMID:16274630]
12. Otsuki, Tetsuji T and 24 more authors..  (2005)  Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries..  DNA research : an international journal for rapid publication of reports on genes and genomes,  [PMID:16303743]
13. Hashiguchi, Masaaki M and 5 more authors..  (2008)  Triggering receptor expressed on myeloid cell-like transcript 2 (TLT-2) is a counter-receptor for B7-H3 and enhances T cell responses..  Proceedings of the National Academy of Sciences of the United States of America,  (29): [PMID:18650384]
14. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,  (5): [PMID:18669648]
15. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,  (16): [PMID:24275569]
16. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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