| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab090652-10μl |
10μl |
现货  |
| |
| Ab090652-50μl |
50μL |
现货 |
| |
| Ab090652-100μl |
100μL |
现货 |
|
| 产品名称 | Recombinant Bax Antibody |
|---|---|
| 别名 | Bax抗体 | anti- Bax抗体 | Bcl2关联X蛋白抗体 | 重组Bax抗体 |
| 英文别名 | Apoptosis regulator BAX antibody | BAX antibody | Bax-protein antibody | BAX_HUMAN antibody | BAXA antibody | Baxdelta2G9 antibody | Baxdelta2G9omega antibody | Baxdelta2omega antibody | Bcl-2-like protein 4 antibody | BCL2 associated X protein antibody | |
| 规格或纯度 | ExactAb™, Validated, Recombinant, High performance, 0.25mg/mL |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | Bax |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 免疫原 | A synthetic peptide of human BAX (AA 1-100). |
| 阳性对照 | WB: A549, HT-29, HeLa, LNCaP, Hep G2 and HKE293 cell lysates. IHC: Human Ovarian cancer tissue. IF/ICC: HeLa cells. Flow: HeLa cells. IP: HT-29 whole cell lysate. |
| 偶联 | Unconjugated |
| 备注 | 本产品即将售完下架，替代产品为 Ab090655 |
| 克隆类型 | 重组抗体 |
|---|---|
| 克隆号 | RR647 |
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3. |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.25mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Recombinant Bax Antibody (Ab090652) - Western Blot
All lanes: Recombinant Bax Antibody (Ab090652) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa
Exposure time: 6.2 s
Recombinant Bax Antibody (Ab090652) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling Bax with Recombinant Bax Antibody (Ab090652) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant Bax Antibody (Ab090652) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant Bax Antibody (Ab090652) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant Bax Antibody (Ab090652) (1/400 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant Bax Antibody (Ab090652) - IP
Bax was immunoprecipitated from 0.5 mg HT-29 whole cell lysate with Recombinant Bax Antibody (Ab090652) at 1/25 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant Bax Antibody (Ab090652) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Bax Antibody (Ab090652) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 30 s.
Recombinant Bax Antibody (Ab090652) - IF/ICC
IF analysis of Recombinant Bax Antibody (Ab090652) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Bax Antibody (Ab090652) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab170148) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 应用名称 | 稀释比例 |
|---|---|
| IP | 1/25 |
| IHC |
1/1600-1/3200
|
| WB |
1/1000-1/2500
|
| Flow Cytometry |
1/100-1/400
|
| IF/ICC | 1:1000 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ25F0319505 |
分析证书 | 25-03-20 | Ab090652 |
| ZJ24F0911347 |
分析证书 | 24-09-27 | Ab090652 |
| ZJ24F0404255 |
分析证书 | 24-04-09 | Ab090652 |
| ZJ23F0901011 |
分析证书 | 23-09-11 | Ab090652 |
| ZJ23F0901012 |
分析证书 | 23-09-11 | Ab090652 |
¥499.90
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