ATG7 Antibody

    应用:
  • IHC
  • WB
功能和特点
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
有货

库存信息

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库存信息

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库存信息

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库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Ab090020-10μl
10μl 期货 Stock Image
Ab090020-50μl
50μL 现货 Stock Image
Ab090020-100μl
100μL 现货 Stock Image
Ab090020-1ml
1ml 期货 Stock Image

基本信息

产品名称 ATG7 Antibody
别名 ATG7抗体 | 自噬相关蛋白7抗体 | ATG7 抗体
英文别名 1810013K23Rik antibody | Apg 7 antibody | APG7 autophagy 7 like antibody | APG7 autophagy 7-like (S. cerevisiae)antibody | APG7 like antibody | APG7, S. cerevisiae, homolog of antibody | APG7-like antibody | APG7L antibody | ATG 7 antibody | ATG12-activat
规格或纯度 ExactAb™, Validated, 0.6 mg/mL
宿主种属 兔(Rabbit)
特异性 ATG7
种属反应性 人(Human)
免疫原 A synthetic peptide derived from human ATG7 (AA 691-703)
阳性对照 WB: Hep G2, THP-1, HeLa, Jurkat and HEK293 cell lysates. IHC: Human colon tissue.
偶联 Unconjugated

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂 0.02% Sodium azide
浓度 0.6 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

ATG7 Antibody (Ab090020) - Western Blot
All lanes: ATG7 Antibody (Ab090020) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 74 kDa
Exposure time: 36.9 s

ATG7 Antibody (Ab090020) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon using ATG7 Antibody (Ab090020). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

关联靶点(人)

ATG7 Tchem 泛素类修饰激活酶 ATG7(Ubiquitin-like modifier-activating enzyme ATG7) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

安全和危险性(GHS)

质量标准

应用

应用名称 稀释比例
WB

1/500-1/1000

IHC

1/50 - 1/100

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

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找到2个结果

批号(Lot Number) 证书类型 日期 货号
ZJ23F1202237 分析证书 23-12-26 Ab090020
ZJ23F1202238 分析证书 23-12-26 Ab090020

参考文献

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2. Buuh ZY, Lyu Z, Wang RE..  (2018)  Interrogating the Roles of Post-Translational Modifications of Non-Histone Proteins..  J Med Chem,  61  (8): (3239-3252).  [PMID:28505447] [10.1021/acs.jmedchem.6b01817]
3. Yuan, W W, Stromhaug, P E PE and Dunn, W A WA..  (1999)  Glucose-induced autophagy of peroxisomes in Pichia pastoris requires a unique E1-like protein..  Molecular biology of the cell,  [PMID:10233149]
4. Tanida, I I, Tanida-Miyake, E E, Ueno, T T and Kominami, E E..  (2001)  The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3..  The Journal of biological chemistry,  (19): [PMID:11096062]
5. Tanida, Isei I, Tanida-Miyake, Emiko E, Komatsu, Masaaki M, Ueno, Takashi T and Kominami, Eiki E..  (2002)  Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p..  The Journal of biological chemistry,  (19): [PMID:11825910]
6. Tanida, Isei I and 6 more authors..  (2002)  Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs..  Biochemical and biophysical research communications,  (22): [PMID:11890701]
7. Sou, Yu-shin YS, Tanida, Isei I, Komatsu, Masaaki M, Ueno, Takashi T and Kominami, Eiki E..  (2006)  Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16..  The Journal of biological chemistry,  (10): [PMID:16303767]
8. Muzny, Donna M DM and 113 more authors..  (2006)  The DNA sequence, annotation and analysis of human chromosome 3..  Nature,  (27): [PMID:16641997]
9. Bechtel, Stephanie S and 18 more authors..  (2007)  The full-ORF clone resource of the German cDNA Consortium..  BMC genomics,  (31): [PMID:17974005]
10. Lee, In Hye IH and Finkel, Toren T..  (2009)  Regulation of autophagy by the p300 acetyltransferase..  The Journal of biological chemistry,  (6): [PMID:19124466]
11. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,  (18): [PMID:19690332]
12. Zhao, Ying Y and 9 more authors..  (2010)  Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity..  Nature cell biology,  [PMID:20543840]
13. Tanida, Isei I, Yamasaki, Manabu M, Komatsu, Masaaki M and Ueno, Takashi T..  (2012)  The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation..  Autophagy,  [PMID:22170151]
14. Bienvenut, Willy V WV and 6 more authors..  (2012)  Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features..  Molecular & cellular proteomics : MCP,  [PMID:22223895]
15. Desai, Shruti S and 8 more authors..  (2013)  Heat shock factor 1 (HSF1) controls chemoresistance and autophagy through transcriptional regulation of autophagy-related protein 7 (ATG7)..  The Journal of biological chemistry,  (29): [PMID:23386620]
16. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,  (16): [PMID:24275569]
17. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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