14-3-3 eta/YWHAH Mouse mAb

    应用:
  • WB
功能和特点
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Mouse IgG
  • 克隆号: 5F2F1E11
  • 偶联: Unconjugated
有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab086596-10μl
10μl 现货 Stock Image
Ab086596-50μl
50μL 现货 Stock Image
Ab086596-100μl
100μL 期货 Stock Image
Ab086596-1ml
1ml 期货 Stock Image

基本信息

产品名称 14-3-3 eta/YWHAH Mouse mAb
别名 14-3-3 eta抗体 | 14-3-3 eta单抗 | 14-3-3 eta鼠单抗 | 14-3-3 eta/YWHAH 小鼠单克隆抗体 | 14-3-3 eta/YWHAH 小鼠单抗
英文别名 1433 eta | 14-3-3 eta | 14-3-3 protein eta | Protein AS1 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide | YWHA1 | YWHA114-3-3 eta | YWHAH | 14 3 3 protein eta antibody | 14-3-3 eta antibody | 14-3-3 protein eta an
规格或纯度 ExactAb™, Validated, 1.2 mg/mL
宿主种属 小鼠(Mouse)
特异性 YWHAH
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 Recombinant protein of human 14-3-3 η (AA 1-514).
阳性对照 WB: HeLa, A431, A549, Jurkat, NIH/3T3 and C6 cell lysates.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 5F2F1E11
Format Whole IgG
亚型 Mouse IgG
SDS-PAGE 150 kDa
纯化方法 Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium azide, pH 7.3
防腐剂 0.02% Sodium azide
浓度 1.2 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

14-3-3 eta/YWHAH Mouse mAb (Ab086596) - Western Blot
All lanes: 14-3-3 eta/YWHAH Mouse mAb (Ab086596) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 54.2 s


关联靶点(人)

YWHAH Tbio 14-3-3 蛋白质(14-3-3 protein eta) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

安全和危险性(GHS)

质量标准

应用

应用名称 稀释比例
WB

1/500-1/1000

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到2个结果

批号(Lot Number) 证书类型 日期 货号
ZJ24F0202780 分析证书 24-02-23 Ab086596
ZJ24F0202781 分析证书 24-02-23 Ab086596

参考文献

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2. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]
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10. Sato, Saori S, Fujita, Naoya N and Tsuruo, Takashi T..  (2002)  Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3..  The Journal of biological chemistry,  (18): [PMID:12177059]
11. Gevaert, Kris K and 6 more authors..  (2003)  Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides..  Nature biotechnology,  [PMID:12665801]
12. Collins, John E JE and 12 more authors..  (2004)  A genome annotation-driven approach to cloning the human ORFeome..  Genome biology,  [PMID:15461802]
13. Yoshida, Kiyotsugu K, Yamaguchi, Tomoko T, Natsume, Tohru T, Kufe, Donald D and Miki, Yoshio Y..  (2005)  JNK phosphorylation of 14-3-3 proteins regulates nuclear targeting of c-Abl in the apoptotic response to DNA damage..  Nature cell biology,  [PMID:15696159]
14. Yang, Xiaowen X and 8 more authors..  (2006)  Structural basis for protein-protein interactions in the 14-3-3 protein family..  Proceedings of the National Academy of Sciences of the United States of America,  (14): [PMID:17085597]
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17. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,  (18): [PMID:19690332]
18. Mi, Lixin and 8 more authors..  (2011)  Identification of potential protein targets of isothiocyanates by proteomics..  Chemical research in toxicology,  (17): [PMID:21838287]
19. Greninger, Alexander L AL, Knudsen, Giselle M GM, Betegon, Miguel M, Burlingame, Alma L AL and DeRisi, Joseph L JL..  (2013)  ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding..  mBio,  (9): [PMID:23572552]
20. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,  (16): [PMID:24275569]
21. Yuasa, Keizo K and 5 more authors..  (2015)  Suppression of death-associated protein kinase 2 by interaction with 14-3-3 proteins..  Biochemical and biophysical research communications,  (14): [PMID:26047703]
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