| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab005387-10μl |
10μl |
现货  |
| |
| Ab005387-50μl |
50μL |
现货 |
| |
| Ab005387-100μl |
100μL |
现货 |
| |
| Ab005387-1ml |
1ml |
期货 |
|
| 产品名称 | Carcino Embryonic Antigen CEA Antibody |
|---|---|
| 别名 | anti- Carcino Embryonic Antigen CEA抗体 | 癌胚抗原多克隆抗体 | 癌胚抗原 CEA 抗体 |
| 英文别名 | Carcinoembryonic antigen antibody | Carcinoembryonic antigen-related cell adhesion molecule 5 antibody | CD66e antibody | CEA antibody | Ceacam5 antibody | CEAM5_HUMAN antibody | DKFZp781M2392 antibody | Meconium antigen 100 antibody | OTTHUMP00000199032 |
| 规格或纯度 | 无载体, ExactAb™, 无叠氮钠, 已验证, 见COA |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | Carcino Embryonic Antigen CEA |
| 种属反应性 | 人(Human),小鼠(Mouse) |
| 交叉反应 | Recognizes human NCA-2 (non-specific cross-reacting antigen-2) |
| 免疫原 | Recombinant CEA expressed in E.coli (AA 448-572) |
| 表位 | Epitope group E as described in Bhayana et al. (1989) |
| 阳性对照 | WB: NIH/3T3 and HeLa cell lysates. IHC: Human Kidney tissue, Human Liver cancer tissue. |
| 偶联 | Unconjugated |
| 克隆类型 | 多克隆抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Antigen affinity purified and Protein A purified |
| 纯度 | ≥95% |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4 |
| 防腐剂 | 0.05% Proclin 300 |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Carcino Embryonic Antigen CEA Antibody (Ab005387) - Western Blot
All lanes: Carcino Embryonic Antigen CEA Antibody (Ab005387) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 70 kDa
Exposure time: 34.5 s
Carcino Embryonic Antigen (Ab005387) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL Carcino Embryonic Antigen (Ab005387)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Carcino Embryonic Antigen (Ab005387) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL Carcino Embryonic Antigen (Ab005387)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 应用名称 | 稀释比例 |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.5-5 µg/mL
|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F0911366 |
分析证书 | 24-09-27 | Ab005387 |
| ZJ23F0901065 |
分析证书 | 23-09-18 | Ab005387 |
| ZJ23F0901064 |
分析证书 | 23-09-18 | Ab005387 |
| 1. Hefta, L J LJ and 5 more authors.. (1990) Expression of complementary DNA and genomic clones for carcinoembryonic antigen and nonspecific cross-reacting antigen in Chinese hamster ovary and mouse fibroblast cells and characterization of the membrane-expressed products.. Cancer research, (15): [PMID:2317824] |
| 2. Schrewe, H H and 8 more authors.. (1990) Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression.. Molecular and cellular biology, [PMID:2342461] |
| 3. Oikawa, S S and 5 more authors.. (1989) Cell adhesion activity of non-specific cross-reacting antigen (NCA) and carcinoembryonic antigen (CEA) expressed on CHO cell surface: homophilic and heterophilic adhesion.. Biochemical and biophysical research communications, (16): [PMID:2803308] |
| 4. Zimmermann, W W, Ortlieb, B B, Friedrich, R R and von Kleist, S S.. (1987) Isolation and characterization of cDNA clones encoding the human carcinoembryonic antigen reveal a highly conserved repeating structure.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3033671] |
| 5. Barnett, T T, Goebel, S J SJ, Nothdurft, M A MA and Elting, J J JJ.. (1988) Carcinoembryonic antigen family: characterization of cDNAs coding for NCA and CEA and suggestion of nonrandom sequence variation in their conserved loop-domains.. Genomics, [PMID:3220478] |
| 6. Beauchemin, N N, Benchimol, S S, Cournoyer, D D, Fuks, A A and Stanners, C P CP.. (1987) Isolation and characterization of full-length functional cDNA clones for human carcinoembryonic antigen.. Molecular and cellular biology, [PMID:3670312] |
| 7. Oikawa, S S, Nakazato, H H and Kosaki, G G.. (1987) Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence.. Biochemical and biophysical research communications, (30): [PMID:3814146] |
| 8. Frängsmyr, L L, Baranov, V V and Hammarström, S S.. Four carcinoembryonic antigen subfamily members, CEA, NCA, BGP and CGM2, selectively expressed in the normal human colonic epithelium, are integral components of the fuzzy coat.. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, [PMID:10436421] |
| 9. Boehm, M K MK and Perkins, S J SJ.. (2000) Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23.. FEBS letters, (9): [PMID:10854848] |
| 10. Taheri, M M and 5 more authors.. (2000) Self recognition in the Ig superfamily. Identification of precise subdomains in carcinoembryonic antigen required for intercellular adhesion.. The Journal of biological chemistry, (1): [PMID:10864933] |
| 11. Ordoñez, C C, Screaton, R A RA, Ilantzis, C C and Stanners, C P CP.. (2000) Human carcinoembryonic antigen functions as a general inhibitor of anoikis.. Cancer research, (1): [PMID:10910050] |
| 12. Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1): [PMID:15057824] |
| 13. Ramachandran, Prasanna P and 5 more authors.. (2006) Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.. Journal of proteome research, [PMID:16740002] |
| 14. Korotkova, Natalia N and 9 more authors.. (2008) Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation.. Molecular microbiology, [PMID:18086185] |
| 15. Govindan, Serengulam V SV, Cardillo, Thomas M TM, Moon, Sung-Ju SJ, Hansen, Hans J HJ and Goldenberg, David M DM.. (2009) CEACAM5-targeted therapy of human colonic and pancreatic cancer xenografts with potent labetuzumab-SN-38 immunoconjugates.. Clinical cancer research : an official journal of the American Association for Cancer Research, (1): [PMID:19789330] |
| 16. Bonsor, Daniel A DA, Günther, Sebastian S, Beadenkopf, Robert R, Beckett, Dorothy D and Sundberg, Eric J EJ.. (2015) Diverse oligomeric states of CEACAM IgV domains.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:26483485] |
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